Browsing by Author "Dayarathna, M.T.A."

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Comparative antibacterial screening on Biophylum sensitivum and Mimosa pudica for diabetic foot ulcer
(Institute of Indigenous medicine, University of Colombo, Rajagiriya, Sri Lanka., 2014) Kularathna, D.P.D.N.; Pushpakumara, A.A.J.; Dayarathna, M.T.A.
Determination of antimicrobial efcacy of Triphala Ashchyotana as a therapeutic agent for acute conjunctivitis.
(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Perera, B.S.R.; Dayarathna, M.T.A.; Perera, B.P.R.; Karunarathne, E.D.C.; Sudesh, A.D.H.
Triphala decoction is a therapeutic agent used in the form of Ashchyotana (eye drops) for acute conjunctivitis. The antibacterial assay was performed according to the standard Antibiotic Sensitivity Test (ABST) for Staphylococcus aureus (ATCC 25923), Escherichia coli, Pseudomonas aeruginosa, Methicillin Resistant Staphylococcus aureus (MRSA) and Candida albicans using chloramphenicol and uconazole as positive controls. The test drug, Triphala decoction, was prepared by boiling dry samples of Terminalia chebula, Terminalia belerica and Phyllanthus emblica 20g each, in 1920ml of water and reducing it to 240ml. Based on the results of ABST, the Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) against S. aureus was determined by using a concentration series ranging from 3 X 50 μl to12 X 50 μl of Triphala decoction with 100μl of S. aureus in 10ml of nutrient broth. The volumes were equalized up to 11ml using sterilized distilled water. After an overnight, incubation at 37OC, 500μl from each concentration was plated using pour-plate technique and incubated overnight at 37OC. To determine the MLC, a loopful of each concentration was streaked on Nutrient Agar plates and incubated overnight at 37OC. According to the results, S. aureus was sensitive to Triphala decoction making an inhibition zone diameter (IZD) of 18mm, while the readings for the other species were in the intermediate range of 17mm for E.coli and 13mm for P. aeruginosa. MRSA strain was resistant to the test drug making IZD 11mm and no inhibition zone was observed for Candida albicans. The MIC and MLC for S. aureus were of the same concentration (6 X 50μl) showing zero growth after that point. It can be concluded that Triphala ashchotana is effective in controlling acute conjunctivitis caused by S. aureus and the dosage required is 6 X 50 μl (6 drops). Triphala can also be used as a prophylactic agent against acute conjunctivitis.
A Phage Indicator Model for In-Vitro Evaluation of Antiviral Drugs Used in Ayurvedic and Traditional Medicine
(19th Conference on Postgraduate Research, International Postgraduate Research Conference 2018, Faculty of Graduate Studies,University of Kelaniya, Sri Lanka, 2018) Dayarathna, M.T.A.; Jayaratne, D.L.
Finding new antiviral drugs for the emerging new viruses have become an outstanding scope of study. The analysis of anti-phage activity evaluation model based on plaque reduction assay has been performed by numerous researchers in evaluating antiviral properties of Western and Ayurvedic medicines. This has been performed by using phages with their corresponding hosts. This study was carried out to validate the antiviral sensitivity of a phage-indicator model to evaluate antiviral drugs used in Ayurvedic and traditional medical treatments in Sri Lanka The host bacterium Escherichia coli was isolated with its corresponding coliphage from sewage effluents. Prior to the antiviral assay, the antibacterial effect of standard antiviral drugs, Acyclovir, Lamuvidine, Ribavarin and Oseltamivir on E. coli was determined. According to the results up to 80mg/ml for Acyclovir, Lamuvidine and Ribavarin did not show any antibacterial effect while Oseltamivir showed only up to 20mg/ml. The antiviral assay was performed in two approaches where the corresponding test drug was applied to a young culture of host bacterium and incubated overnight prior to the inoculation of the bacterium by phage suspensions. As the second approach, the test drug was applied to a phage suspension and incubated overnight prior to the inoculation of phage into the bacterium. According to the reduction of plaques on agar plates after performing the two approaches the mode of antiviral activity was determined. There was a significant reduction of plaque formation observed when the host bacterium was treated with the western antiviral drugs, suggesting that their antiviral effects occur during the viral replication stages in the host cell and this complies with the available literature which describes the mode of action of these Western antiviral drugs. The model was also used to evaluate traditional herbal extracts to determine the presence of antiviral properties. Accordingly, the second approach revealed that the aqueous black tea extract showed an antiviral effect during the adsorption stages while crude papaya leaf extract in the replication stages of the virus life cycle. However, the Ayurvedic product ‘Sudarshana Churnaya’ showed no effects in either the two stages of the virus life cycle. With these results it is concluded that this model is feasible, and efficient against laborious cell culture techniques in screening antiviral drugs
Potential Biocide Options and Biological Control Agent For Ceratocystis paradoxa Isolated From Coconut Growing Areas of Sri Lanka
(2016) Jayaratne, D.L.; Dayarathna, M.T.A.
A study on antibacterial and antifungal effect on Rhinacanthus nasutus Linn. (Aniththa)
(Gampaha Wickramarachchi Ayurveda Institute, University of Kelaniya, 2014) Dayarathna, M.T.A.; Jayarathna, D.L.