Browsing by Author "Jayasekara, D."
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Item Comparative Analysis of Albumin Measurement by Bromocresol Green Versus Capillary Zone Electrophoresis(College of Chemical Pathologists of Sri Lanka, 2024) Harshanee, T.; Jayasekara, D.; Fernando, K.; Kulasinghe, M.; Silva, P.; Bandara, S.; Dayanath, B.K.P.T.INTRODUCTION Quantitative assessment of albumin and globulins in serum protein electrophoresis (SPE) relies on the calculation derived from total protein measurements obtained from the biuret assay. Validation of these results involves comparing sample albumin concentration assessed using the bromocresol green (BCG) method to those obtained from capillary zone electrophoresis (CZE). This study aimed to assess the relationship between albumin values derived from SPE and routine spectrophotometric analysis with BCG method, and to establish a cut-off value to identify significant differences between the two values in SPE validation process. METHODS 187 serum samples were subjected to total protein assessment by biuret method, albumin assessment by BCG method, and SPE by CZE method. SPE derived albumin level using total protein values and BCG method indicated albumin values were analyzed by correlation analysis. The standard error for the difference (SEdif) between the two albumin results were calculated, with statistical significance set at p <0.05. Internal and external quality assessments were deemed acceptable for all three analytical procedures. RESULTS Mean albumin levels obtained via BCG and CZE methods were 38.3 mg/dL and 33.2 mg/dL, respectively, indicating a negative bias for the latter method. A strong correlation (r=0.844, p<0.001) was observed between albumin levels measured by BCG and CZE methods, with Bland-Altman analysis revealing an average negative bias of 5 mg/dL. Using SEdif at 95% significance, the maximum acceptable difference between the two albumin values was determined to be -5.4 mg/dL. CONCLUSIONS BCG method yielded higher albumin results compared to CZE method. This positive bias could be attributed to potential lack of specificity of BCG assay for albumin. A maximum allowable difference of 5.5 mg/dL between the two methods is suggested. Exceeding this threshold indicates potential issues with either the BCG albumin assay, biuret total protein assay, or SPE assay, necessitating further investigation for assay reliability, particularly in reporting SPE by CZE.Item Comparative analysis of alkaline phosphatase with two assays using different buffers; diethanolamine (dea) and 2-amino-2-methyl-1-propanol (amp): establishing correlation factors for diagnostic consistency(College of Chemical Pathologists of Sri Lanka, 2024) Jayasekara, D.; Fernando, K.; Kulasinghe, M.; Silva, P.; Madurangi, D.W.D.D.; De Silva, D.D.S.; Harshanee, K.G.A.T.; Bandara, S.R.R.; Dayanath, B.K.T.P.INTRODUCTION AND OBJECTIVES Alkaline phosphatase (ALP) serves as a pivotal biomarker for bone and liver diseases, employing assays utilizing either 2-amino-2-methyl-1-propanol (AMP) (IFCC recommended) or diethanolamine (DEA) buffers, with the latter consistently yielding higher values. This study aimed to develop a correlation factor for ALP reagents using DEA buffer from supplier X, in comparison to routine automated ALP assay at the central laboratory using AMP.METHODS Twenty-five serum samples were analyzed in the central laboratory assay using AMP buffer in a fully automated analyzer with dedicated reagents and the test assay using DEA buffer on a semi-automated biochemistry analyzer within two hours of receipt. Both assays employed the same biochemical reaction, differing only in buffer composition. The linearity ranges for the test assay with DEA buffer and the routine assay with AMP buffer were determined as 1600 U/L and 800 U/L, respectively. RESULTS Patient samples exhibited ALP levels ranging from 0 to 339 U/L by routine assay. The correlation graph demonstrated a satisfactory R2>0.75, indicating adequate number of sample inclusion and quality. A correction factor of 1.2 was calculated for the ALP assay utilizing DEA, compared to the AMP-based assay, employing simple linear regression analysis.CONCLUSIONS According to the sample availability, only ALP levels up to 339 U/L by AMP-based assay were included. Therefore, the correction factor of 1.2 is applicable only up to an ALP level of 400 U/L with the DEA-based assay, necessitating dilution of samples with higher values for the correlation factor’s application. This study indicates a correction factor of 1.2, which is deviated from factors close to 2, observed in literature because of reagents being from different manufacturers and running two assays on two different platforms (automated/ semiautomated). It is important to derive a factor for an ALP assay with DEA buffer to make the results comparable to IFCC recommended AMP buffer used ALP assay.Item Evaluation of point-of-care testing (poct) devices for cardiac troponin i in screening patients with myocardial infarction(College of Chemical Pathologists of Sri Lanka, 2024) Fernando, K.; Jayasekara, D.; Kulasinghe, M.; Silva, P.; Harshanee, K.G.A.T.; Bandara, S.R.R.; Dayanath, B.K.T.P.INTRODUCTION AND OBJECTIVES Cardiac troponin I (cTnI) is a crucial biomarker for diagnosing myocardial infarction (MI). However, many remote hospitals lack access to cTnI assessment facilities. This study investigates the feasibility of using Point-of Care Testing (POCT) devices to triage MI patients in such settings, facilitating their transfer to tertiary care hospitals. Assessing the quality of POCT devices is essential for this purpose. This report outlines the assessment methodology of two POCT devices and presents the obtained results.METHODS Two POCT devices, labelled X and Y, for measuring cTnI were compared against the Ortho-Vitros 3600, serving as the reference method, with optimum internal and external quality control measures. Basic specifications of the POCT devices and the comparator were obtained from their respective kit inserts. Routine patient samples were analyzed in singlicate using POCT devices and the reference method. Linear regression analysis was conducted, and correlation graphs were generated. Within-run precision was evaluated using a patient sample and imprecision (CV) was calculated for the POCT devices.RESULTS The linearity ranges for cTnI measurement with POCT devices X and Y were 0.01–15 ng/mL and 0.03–30 ng/ mL, respectively, with decision-making cutoff values for diagnosing MI established at 0.04 ng/mL and 0.5 ng/ mL, respectively. Regression analysis demonstrated acceptable linearity for both POCT devices, with correlation of R2=0.7388 for device X and R2=0.8881 for device Y. However, higher imprecision was observed for both analyzers, with a CV of 26% for device X and 20% for device Y at decision-making cutoff levels.CONCLUSIONS This study highlights major challenges associated with the implementing POCT devices for cTnI assays in triage settings aimed at diagnosing MI in acute care. Specifically, the adoption of higher cutoff levels in the POCT devices, not aligned with the recommended 99th percentile for the healthy population, and the poor precision observed at these cutoff values emerge as significant hurdles.Item Intercultural influences on the architectural traditions of Jaffna Peninsula: a study focused on the architectural remains of the Manthiri Manai(University of Kelaniya, 2013) Herath, H.M.D.B.; Somarathne, M.P.D.S.; de Pasqual, M.K.; Jayasekara, D.Architecture occupies a special place in rediscovering the early cultural traditions of the Jaffna peninsula. Architectural traditions of the Jaffna Peninsula which was routed in the south Indian traditions of Kerala and Tamil Nadu were subjected to change under the impact of western influences. The aim of this paper is to investigate the architectural traditions of the Jaffna peninsula which is a window for Tamil national culture. The ancient South Indian and Kerala architectural traditions are best illustrated by the household buildings, religious buildings and other public buildings. However, this original traditional architecture has been overshadowed by Dutch influences. Present day architecture of Jaffna peninsula can be seen as an admixture of both the past as well as the modern influences.Item Pulathisi Ravaya: The Lost Opportunity in Rural Broadcasting(Faculty of Graduate Studies, University of Kelaniya, Sri Lanka, 2016) Herath, D.B.; Rupasinghe, W.; Rupasinghe, W.; Jayasekara, D.; Aththanayaka, M.Sri Lanka is one of the first countries which started radio broadcasting in the early 20th century. This broadcasting unit under the British colonial rule was maintained for the benefit of the rulers to entertain their officials and the troops located in several areas of the Indian sub-continent. After the end of the Second World War radio began to air more local content since independence radio broadcasting continued as a government department. 1979 was the year in which the Colombo centered broadcasting monopoly ended with the opening of the Rajarata Sevaya as a first regional Radio Broadcasting Service. Since then few stations like Ruhuna and Kadurata started, they inaugurated another format of radio broadcasting popularly named “Community radio”. Pulathisi Ravaya was yet another Broadcasting service which was started in 1995 under the control of SLBC and was maintained by the Mahawali Development Authority. It functioned until 2002 and terminated without any known legitimate reason. This study tries to analyze Pulathisi Ravaya by using the case study method. For collecting data, we have deployed several methods such as field study, content analysis of the programme scripts, interviews and primary and secondary sources such as administrative reports and reminiscences of the broadcasters. We found that this well-established radio service terminated due to lack of cooperation, misunderstanding and the power struggle between the stakeholders of the radio. This study suggests that exploring the possibilities of re-inaugurating it and filling the vacuum for radio broadcasting like Pulathisi Ravaya in the North Central ProvinceItem Radio “Dambana”: A new experiment of Community radio in Sri Lanka(Faculty of Graduate Studies, University of Kelaniya, 2015) Herath, D.B.; Rupasinghe, W.; Jayasekara, D.; Gamage, D.; Attanayaka, M.Sri Lanka is the first country which started the Community Radio station in Asia, as Mahawali Community Radio (MCR).But many researchers points out that it was not a proper way of establishing a community radio.(Jayaratne,2008).The rationale behind is that its name as ―community‖ but functioned as ―state owned‖. In 2010 the main MCR station Giradurukotte Community Radio, launched as Radio Dambana. It was transformed as community radio for Sri Lankan Vadda community mainly located in the area of Dambana. In this research we are trying to analyze the main functions of the radio dambana as a community radio. While doing this we are searching the sustainability of the station and its scope. We have used mix methods to collect data such as interviews field visits, content analysis of the programmes as primary data. To analyze the data we deployed the SWOT analysis as a tool. The findings suggest the format of radio dambana would need a shift of its functions to achieve the aims and objectives of the station.Item A study of the application of the Community Radio in the Sri Lankan Perspective; Based on Kothmale Community Radio(Research Symposium 2010 - Faculty of Graduate Studies, University of Kelaniya, 2010) Jinadasa, W.W.M.P.K.; Rajapaksha, S.; BandaraHerath, D.; Somorathne, D.; Jayasekara, D.The objective of this study is to analysis of the impact of the Kothmale Community Radio towards its community. Problem is that why does community radio not function properly and what are the consequences that lead to collapse the Community radio. The Hypothesis The principle of the community radio has not been appropriately established. This study was carried out during the period of in 2010. Methodology has consisted both quantitative and qualitative approaches. In this, it was used survey study consisting 250 questionnaires, for Kothmale and villages which are closely situated behind the community radio. Participatory observation and interviewing were used as the qualitative tools. Findings reveal that at the early stage there was a great impact upon its community. In comparison to its beginning there is no consequential participation of the rural community for the radio and in return no greater advantage upon the rural mass at present. Nowadays, the impact of the national radio channels has been a wide practice rather community radio. No community participation as emphasized in its principle for the development of the community while, bigger political interferences which are channeled through the top to down political higherachy.Item රූපවාහිනී මාධ්ය තුළ විකාශය වන වාස්තු විද්යාත්මක වැඩසටහන්වල ස්වභාවය පිළිබඳ අධ්යයනයක්(Faculty of Social Sciences, University of Kelaniya, Sri Lanka, 2015) Fernando, W.H.E.D.; Jayasekara, D.Item ෆේස් බුක් භාවිතයේදි අනුගමනය කළ යුතු ආරක්ෂක ක්රියාමාර්ග පිළිබඳ පරිශීලකයන් දැනුවත්ද යන්න විමර්ශනය(Faculty of Social Sciences, University of Kelaniya, Sri Lanka, 2015) Manamperi, M.M.S.P.; Jayasekara, D.