Browsing by Author "Jayasiri, A.P.A."

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Estimation of total phenolic content on stem bark extracts of selected Sri Lankan medicinal plants
(National Centre for Advanced Studies in Humanities and Social Sciences Sri Lanka, 2015) Jayasiri, A.P.A.; Paranagama, P.A.; Senanayake, S.P.; Amarasinghe, A.P.G.
Phenetic analysis and phytochemical screening of Albizia lebbeck and its substitute plants in Sri Lanka
(University of Kelaniya, 2013) Jayasiri, A.P.A.; Senanayake, S.P.; Paranagama, P.; Amarasinghe, A.P.G.
Albizia lebbeck (vern: Suriya mara) is a tree belonging to the family, Fabaceae. It is native to tropical Southern Asia, and found widely in India. It has been used in traditional therapeutic systems of Ayurveda, Sidhdha and Unani, for preparation of drugs for many diseases. Due to the limited distribution of A. lebbeck in Sri Lanka, substitute plants are used in the drug manufacturing industry. A questionnaire survey was carried out on a sample population of hundred Ayurvedic physicians, traditional Ayurvedic practitioners, drug suppliers, drug manufacturers and the general public, in order to collect traditional knowledge in the medicinal uses of these plants. The survey revealed that A.odoratissima, Adenathera pavonina and Samanea saman are commonly used substitute plants for A. lebbeck in traditional medicinal systems. To evaluate the phenetic diversity, a morphometric study and cluster analysis were carried out using floral and vegetative characters of A. lebbeck and its substitute plants. Variations in the bark, inflorescences, floral colour and type, texture and colour of pods are found as the important diagnostic characters of these species. Cluster analysis clearly indicated the morphological variation in population samples of all four plant species. Stem bark of the four species were subjected to sequential solvent extraction using hexane, chloroform, methanol and water. Weights of each crude sample were obtained after evaporation of the solvent. Highest yield was obtained from the methanolic extracts which revealed the presence of polar compounds in the species. The extracts were subjected to the preliminary phytochemical screening for carbohydrates, proteins, amino acids, glycosides, tannins, phenolics, alkaloids and saponins, Phytochemical analysis has confirmed the presence of glycosides, flavonoids, tannin, phenolics and phytosteroids in methanolic extracts of the four species. The results of the present study reveal the presence of saponin, tannins and phenolic compounds in the water extracts of the four plants. The distribution of classes of phytochemicals in the four plants was similar to each other except alkaloids as it was found only in Adenathera pavonina and Samanea saman. Therefore, this study has provided supportive evidence for the possibility of the presence of similar medicinal properties in A. lebbeck, A.odoratissima, Adenathera pavonina and Samanena saman. These findings can be considered as valuable facts in the recommendation of the use of these three plants as substitutes of A. lebbeck in medicinal preparations. Further investigation on the similarity in bioactivity of the four plants is needed to confirm this recommendation.
Phenetic analysis and phytochemical screening of medicinally important Albizia spp. in Sri Lanka
(Ceylon Journal of Science (Biological Sciences), 2016) Jayasiri, A.P.A.; Senanayake, S.P.; Paranagama, P.; Amarasinghe, A.P.G.
Albizia Durazz. is a genus of 150 species in the tropics and subtropics of the world and belongs to the sub family Mimosaceae in the Family Fabaceae. Of the six species recorded in Sri Lanka, A. lebbeck is used as a shady tree while A. odoratissima is grown mainly for their timber value, However, A. odoratissima and A. lebbeck are found to be used in ayurvedic medicine however, the medicinal properties of these species are not fully understood. A questionnaire survey was carried out using a hundred sample population to identify their medicinal usage. Floral and vegetative characters of the above two Albizia spp. were observed and phenetic relationships were identified. Air dried stem barks of A. odoratissima and A. lebbeck were subjected to sequential solvent extraction using hexane, chloroform, methanol and water, and the crude weight of the yield were obtained. The results revealed that ayurvedic physicians and traditional ayurvedic medical practitioners use A. odoratissima in medicinal preparations whereas the medicinal use of A. lebbeck is not reported. Further, it was revealed that Samanea saman and Adenanthera pavonina are commonly used as substitutes for A. lebbeck. Knowledge of phenetic variation of the two Albizia spp. can be used for accurate identification which prevents adulteration. Highest yield was obtained from the methanolic extracts. These extracts were subjected to preliminary phytochemical screening to assess the occurrence of different phytochemicals. Results have shown the presence of glylcosides, tannins, phenolics, phytosteroids and flavonoids in methanolic extracts A. odoratissima, and A. lebbeck. The present study suggests that further studies should be conducted on the identification of active compounds in these two plant species for their pharmacognostic properties in order to understand their mode of remedial action for ailments.
Phytochemical screening and In vitro anti-inflammatory activity of stem bark of Samanea saman
(National Centre for Advanced Studies, 2014) Jayasiri, A.P.A.; Paranagama, P.A.; Senanayake, S.P.; Amarasinghe, A.P.G.
Phytochemical screening of Albezia odorantissima stem bark
(Institute of Indigenous Medicine, University of Colombo, 2014) Jayasiri, A.P.A.; Paranagama, P.A.; Senanayake, S.P.; Amarasinghe, A.P.G.
Preliminary phytochemical screening of the medicinal Plant Adenanthera pavonina and its morphological variation
(Sri Lanka Journal of Indegenous Medicine (SLJIM), 2013) Jayasiri, A.P.A.; Senanayaka, S.P.; Paranagama, P.A.; Amarasinghe, A.P.G.
Adenanthera pavonina L.(vernacular:Madatiya) is a medicinal plant, belongs to family Fabaceae. It is widely used for the treatments of many diseases in various therapeutic systems including Ayruveda therapeutic system. The medicinal uses and the distribution of the species were studied using a structured questionnaire survey considering 100 sample population. Morphological characters were analyzed using the specimens collected from their natural habitats to infer the phenetic relationships and has shown no variations with respect to their habitat differences. Qualitative phytochemical analysis was done to identify the chemical compounds present in the stem bark extracts of different solvents such as hexane, chloroform, methanol and water. Phytochemical screening of stem barks of the different samples confirms the presence of phytochemicals; alkaloids, flavonoids, glycosides, tannins, steroids, proteins and saponins in the extracts of the methanolic, chloroform and water. This study draws attention to the need of further analysis of the active principles of the species in order to understand their mode of action in controlling different diseases.
Taxonomic and Phytochemical Study on Albizia lebbeck and Substitute Plants used in Ayurvedic Drug Preparations in Sri Lanka
(University of Kelaniya, 2012) Jayasiri, A.P.A.; Senanayake, S.P.; Paranagama, P.; Amarasinghe, A.P.G.
Nowadays consumption of herbal medicines is widespread and has increased dramatically. The main supply of herbal material for ayurvedic drug preparations is from the wild. It causes inherent problems: misidentification, phenotypic variability, extract variability and adulteration. The pharmacognostic evaluation is the preliminary step in the standardization of crude drugs which provides valuable information in morphology and physical characteristics, and the purity and quality of the plant drugs. Albizia lebbeck (Sinhala- Mara, Sanskrit-Mahari, Hindi- Shiris ) is a South Asian medicinal plant widely cultivated and naturalized in tropical and subtropical regions. In Sri Lanka many substitute plants are used due to the restricted distribution which has caused ambiguity in utilizing accurate plant material. The present study focuses on exploring the use of A. lebbeck and its substitutes in ayurvedic drug preparations with reference to their morphological and pharmacognostic similarity. A questionnaire survey was carried out, using a randomly selected sample population of 100, to determine the use of substitute plants and it revealed that three plants in the subfamily Mimosodeae, Albizia odoratissimma (Sinhala-Suriya mara), Adenanthera pavonina (Sinhala-Madatiya) and Samanea saman (Sinhala-Pare mara) are used widely in Sri Lanka, whereas A. odoratissima is being predominantly used in drug preparations. Specimens of the above plants were collected from the natural habitats and indentified using the authenticated specimens at the National Herbarium. Methanolic bark extracts of A. odoratissimma and A. pavonina were subjected to a preliminary phyotochemical screening to detect the different secondary metabolites, such as carbohydrates, proteins, amino acids, glycosides, and alkaloids. Further, phytochemical screening was carried out using solvents; ethyl acetate, methanol and water. Thin Layer Chromatography was performed on each extract, for the qualitative and quantitative analysis. High yield was obtained from methanolic extracts that indicated the presence of polar compounds. Chromatographic properties have showed the variation of chemical profiles in these two bark extracts. These compounds will be characterized by fractional analysis, and their distributional patterns in these plant species will be compared to evaluate the effectiveness as substitutes to A. lebbeck.
Taxonomic study on Albizzia lebbek and its substitute plants used in Ayurvedic preparations in Sri Lanka
(University of Kelaniya, 2011) Senanayake, S.P.; Jayasiri, A.P.A.
Consumption of herbal medicines is widespread and increasingly used nowadays. The main supply of herbal materials for ayurvedic preparations is from the wild, which causes inherent problems; for instance, misidentification, phenotypic variability, extract variability and contamination. Albizzia lebbek (Sin- suriyamara, San- mahari, Hin- siris) is a South Asian medicinal plant, widely cultivated and naturalized in tropical and subtropical regions. It is extensively used in Ayurvedic preparations in India whereas many substitute plants are used in Sri Lanka due to the restricted distribution. This has caused ambiguity in utilizing accurate plant material in Sri Lanka. The present study focused to explore the use of Albizzia lebbek and its substitutes in Ayurvedic preparations with reference to their morphometric similarity and geographical distribution. A survey carried out to determine the use of substitute plants through a sample survey questionnaire revealed that three plants in the subfamily Mimosoideae; Albizzia odoratissima (Sin- huriya mara), Adenanthera pavonina (Sin- madatiya) and Samanea saman (Sin- pare mara) were common and widespread, Albizzia odoratissima being predominantly used. The randomly selected sample population of one hundred included traditional Ayurvedic practitioners, Ayurvedic physicians, drug dealers/ suppliers, drug manufacturers and the general public. Further, Albizzia lebbek was recorded only in a few locations in Sri Lanka and use of this plant in Ayurvedic preparations is not reported. A comparative morphometric analysis of these plants was done based on seedling, leaf, bark, floral, pod and seed characters and multi-access key was constructed using DELTA (Description Language for Taxonomy) package which could serve as a pictorial guide for accurate identification.
Taxonomy and pharmacognostic properties of Albizia lebbeck and substitute plants in Sri Lanka
(2016) Jayasiri, A.P.A.