Browsing by Author "Karunarathna, E.D.C."

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An analytical study on the antimicrobial activity of Visarpahara Tailaya against Candida albicans and Staphylococcus aureus.
(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Madumali, M.G.B.; Wickramarachchi, W.J.; Karunarathna, E.D.C.; Sudesh, A.D.H.
Visarpahara Tailaya is a herbo-mineral preparation recommended to use clinically in the management of eczema, scabies, itches and other types of skin diseases mainly caused by bacteria and fungi. This study was conducted to determine the antimicrobial activity of this drug using the standard Antimicrobial Sensitivity Test against Staphylococcus aureus and Candida albicans. The test was performed according to the Well diffusion Method having 6 mm diameter wells on Mueller Hinton Agar (MHA) for S. aureus and Sabouraud Dextrose Agar (SDA) for C. albicans. As the positive controls, Amoxicillin 10mg/ml was used for S.aureus and Fluconazole 2.5mg /ml for C. albicans. According to the results, the test drug showed an average Inhibition Zone Diameter (IZD) of 12mm for S. aureus and 17mm for C. albicans while the positive controls showed 51mm and 25mm respectively .These results show that the test drug Visarpahara Thailaya is effective for both test organisms with an increased effectiveness for C.albicans compared to S. aureus. Therefore, it is concluded that Visarpahara Tailaya can be effectively used in the management of C. albicans while it is less effective for S. aureus.
Antibacterial activity of extract of leaves of Atalantia ceylanica (Yakinaran)
(Faculty of Graduate Studies, University of Kelaniya, 2015) Munasinghe, D.A.L.; Karunarathna, E.D.C.; Sudesh, A.D.H.
Antibacterial activity of leaves of Atalantia ceylanica was tested qualitatively against 05 bacterial strains with the modified plate method at Gampaha Wickramarachchi Ayurveda Institute. The steam of boiled leaves and extract are used by certain villagers as a traditional treatment for healing respiratory ailments such as influenza. Even though natural flora may be effective in curing health defects, scientific studies on subject is limited. Thus the study was arranged to fill the gap for certain extent to harness natural resources effectively in health. So as the study was aimed to investigate the antibacterial properties of Atlanta leaves. The plates, prepared with the watery extract of boiled leaves, which comprised of neat concentration of raw leaf materials and Mueller-Hinton agar were inoculated with laboratory stored standard bacterial strains including Pseudomonas aeruginosa, Streptococcus pneumonia, Escherichia coli, Staphylococcus aureus and Methicillin resistant Staphylococcus aureus separately, which can cause respiratory as well as gastro- intestinal symptoms. For the control test, distilled water was used to make plates instead of watery leaf extract. Even after the duplicated experiment, no strain was found to be inhibited by the leaf materials, which indicated that the neat concentration of raw chemical compound of particular leaves have no inhibitory action on tested strains. As the respiratory infection is not only caused by bacteria but also by viruses, perhaps phytochemicals might have possessed antiviral as well as antibacterial properties on organisms other than the tested strains. Occasionally the chemicals of the particular plant leaves might have possessed certain antiinflammatory features to control respiratory symptoms. Nevertheless it was clear that the chemical compounds of the distillation of leaves of Atalantia ceylanica had no significant inhibitory effect on the growth of tested strains. The same experiment was carried out early for the steam of particular leaf and the results were found to be the same.
A comparative study on the anti-microbial efficacy of two Ayurveda powders (Prathisarana) used in periodontal diseases
(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Wijebandara, D.M.K.K.; Peiris, K.P.P.; Karunarathna, E.D.C.; Sudesh, A.D.H.
Kushtadi (KU) and Karanjadi (KR) are prominently used Ayurvedic powders (Prathisarana) in the management of periodontal diseases. This study was carried out for the determination of antimicrobial efcacy of these two drugs using standard Antimicrobial Sensitivity Test against Candida albicans and a fungal culture isolated from a patient. The Antimicrobial Sensitivity Test was performed according to the Well diffusion method having 6mm diameter wells on Sabouraud Dextrose Agar (SDA). Each well was loaded with test drugs mixed in 20μl of sterile distilled water and uconazole 2.5mg / ml as the positive control. According to the results, KR showed an average Inhibition Zone Diameter (IZD) of 20mm for the isolated culture while KU did not show any inhibition. The results were similar for Candida albicans, KR showing IZD 18 mm, KU IZD 0 mm while positive control showed an inhibition of 25 mm. Therefore, it can be concluded that both Candida albicans and the isolated culture were sensitive only for KR making it the only effective drug out of the two drugs tested in this study.
Microbiological study of an indigenous mouth wash (Gandusha) used in periodontal diseases.
(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Dewasurendra, D.M.W.; Peiris, K.P.P.; Karunarathna, E.D.C.; Sudesh, A.D.H.
Mouth wash (Gandusha) is clinically effective in the management of periodontal diseases. This study is an in-vitro analysis on determining the antimicrobial efcacy of this drug using standard Antimicrobial Sensitivity Test against a fungal culture isolated from a patient. The test was performed according to the Well diffusion method having 6mm diameter wells on Sabouraud Dextrose Agar (SDA). Each well was loaded with the test drug mixed in 20μl of sterile distilled water and uconazole 2.5mg / ml was used as the positive control. According to the results, the test drug showed an average Inhibition Zone Diameter (IZD) of 19mm while the positive control showed IZD of 15mm. Therefore, it can be stated that the test drug is more effective than the positive control and the isolated culture is sensitive to the test drug as it had shown an IZD of 19mm while it is intermediate sensitive for the positive control as 15mm comes under Intermediate sensitive range (15-18mm). These results support the claim that the indigenous mouth wash (Gandusha) is effective in the management of periodontal diseases.