Browsing by Author "Mahalingam, S."

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Application of Elisa in the diagnosis of rotavirus infections in buffalo calves
(An International Journal Of Buffalo Science., 1994) Sunil-Chandra, N.P.; Mahalingam, S.
The conditions for diagnosis of group A rotavirus infection in buffalo calves by enzyme linked immunosorbent assay (ELISA) were optimised in terms of type of microtitre plates, all reagents and the cut off points for positivity. Irradiated polystyrene plates were the plates of choice. The optimal dilution for the clinical samples (faecal extracts), capture and detector antibodies and the enzyme conjugate were I : 10, I : 5,000, I : 10,000 and I : 300, respectively. Further, we found that the cut off point for positivity by the screening ELISA was an optical density (OD) of ≥ 0.170 at 450 nm wave length, and for confirmation when blocking activity was ≥ 30%. 'The positivity of a faecal sample was graded as strongly positive if the PIN value was ≥2.7 by screening ELISA and ≥ 50% blocking activity in the confirmatory blocking ELISA. Samples having PIN value < 2.7 but ≥ 2. I and < 50% but ≥ 30% blocking activity were regarded as weakly positive. in addition, pre and post colostral buffalo sel'd as negative and positive control sera respectively, were selected and used for detection of antirotavlral antibodies by the blocking ELISA. 'This study establishes that the ELISA technique can be profitably used (once required parameters are defined), in the diagnosis of rotavirus infection in buffalo calves.
Drivers of Customer-based Brand Equity in Personal Care Products in Coimbatore City
(International Conference on Business and Information (ICBI – 2019), [Marketing Management], Faculty of Commerce and Management Studies, University of Kelaniya, Sri Lanka, 2019) Mahalingam, S.
The purpose of the study is to determine the relationship between the Socio-economic factors with the brand of personal care products. The study is mainly based on primary data which was collected from 492 customers. This study develops and empirically tests a model for Customer - Based Brand Equity among Personal Care Products. Statistical tools such as Karl Pearson's coefficient of correlation and multiple regression analysis were used to analyze the data. The dimensions of brand equity such as brand awareness, brand knowledge, perceived quality, brand association, purchase decision and, post-purchase behavior were examined through various tools. Results of the research are focused on the Customer-Based Brand Equity and discussed by considering the perception of customers with regard to attributes and benefits, purchase decision, awareness, association, identity, knowledge, performance and loyalty in fetching.
Isolation and subgrouping of rotaviruses from buffalo calves in Sri Lanka
(British Veterinary Association, Elsevier, 1996) Sunil-Chandra, N.P.; Mahalingam, S.
Twenty-eight faecal specimens from Sri Lankan buffalo calves shown to be positive for rotavirus group A antigen were subgrouped by an enzyme-linked immunosorbent assay, by using monoclonal antibodies prepared against subgroup I and II antigens. The 13 of the 28 specimens which were classified as strongly positive belonged to subgroup I. Three of five faecal specimens inoculated on to roller cultures of MA104 cells yielded group A subgroup I rotavirus. As with other group A rotaviruses isolated from human beings and young animals, the buffalo isolates required pre-treatment with trypsin and to have trypsin incorporated in the maintenance medium, and the inoculated cell cultures had to be rolled; at least six serial passages were required before distinct rotavirus cytopathic effects were produced in the MA104 cells.
Rotavirus-associated diarrhoea in buffalo calves in Sri Lanka
(British Veterinary Association, Elseveir, 1994) Sunil-Chandra, N.P.; Mahalingam, S.
Faecal samples from 150 buffalo calves, one to 150 days old, located in various districts of Sri Lanka, were examined for group A rotavirus antigen by a screening enzyme linked immunosorbent assay (ELISA). Positive samples were confirmed by the blocking ELISA. In the calves studied 27.3 per cent were diarrhoeic, and the rest were non-diarrhoeic but were in contact with the animals showing diarrhoea. Antigen was detected in 36.6 per cent of the diarrhoeic animals and in 11.9 per cent of the non-diarrhoeic animals. There was a strong association between the presence of antigen in faeces and diarrhoea in these animals (chi 2 = 46.98; P < 0.001). Of the 146 serum samples examined for antirotaviral antibodies, by the blocking ELISA at a single serum dilution (1:20) against a constant dose of antigen (8 units), 68.5 per cent were positive indicating a widespread infection with the virus in the population studied. This is the first record of the detection of rotavirus and its association with diarrhoea in buffalo calves in Sri Lanka.
Study of natural rotavirus infection in buffalo calves in Sri Lanka
(Springer, 1995) Sunil-Chandra, N.P.; Mahalingam, S.
No Abstract Available