Browsing by Author "Prabodha, L.B.L."

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Anatomical dimension of the Caudate lobe of the liver
(University of Kelaniya, 2011) Widanagama, M.A.; Prabodha, L.B.L.; Palahepitiya, K.N.; Nanayakkara, B.G.; Ilayperuma, I.; Hasan, R.
On the posterior surface of the liver, the Caudate lobe lies between the inferior vena cava and the fissure for the ligamentum venosum. It is connected to the right lobe of the liver by the Caudate process. However, functionally it belongs to the left lobe. In left hepatic lobectomy, the left lobe together with most of the Caudate lobe is removed. Furthermore, the diameters of right lobe and Caudate lobe have been used to distinguish between the normal and cirrhotic livers. This study was carried out to establish the anatomical dimensions of the Caudate lobe in a group of adult Sri Lankans. A total of 26 apparently healthy livers obtained from formalin fixed cadavers were studied. The Caudate lobe was measured along its maximum antero-posterior and transverse diameters using a sliding caliper capable of measuring to the nearest 0.01 mm. Maximum transverse diameter of the right lobe of the liver was taken to compare the ratio between the right lobe and the Caudate lobe. The mean maximum antero-posterior and transverse diameters of the Caudate lobe was 51.6 ± 4.6 mm and 27.6 ± 4.5 mm respectively. The transverse diameter of the right lobe was 85.05± 13.2 mm. The mean ratio of the transverse diameter of the Caudate lobe to that of the right lobe was found to be 0.32 ± .06. The results of the study demonstrate the anatomical dimensions established for the Caudate lobe for a group of adult Sri Lankan population. These data will facilitate in improving the outcome of surgical procedures of the liver.
Evaluation of the clinical features and identification of 22q11 deletion among selected patients with cleft palate
(University of Kelaniya, 2013) Prabodha, L.B.L.
Cleft palate is one of the commonest congenital malformations of the head and neck region. The incidence of isolated cleft palate in the central province of Sri Lanka was 0.19 per 1000 live births. Micro-deletion of chromosome 22qll.2 is one of the commonest syndromic causes of cleft palate. Objective of the study is to describe the socio demographic background, clinical features and prevalence of the 22qll.2 deletion among patients with cleft palate in Sri Lanka. Patients with cleft palate without cleft lip attending the Regional Cleft Centre & Maxillo-Facial Department, Teaching Hospital, Karapitiya were clinically assessed and semi-quantitative PCR was performed to identify the 22ql 1.2 deletion. There were 162 subjects (males 41.36%) with an age range of two weeks to forty nine years. Majority had clefts involving the soft palate (n=125/162; 77.16%). There was a statistically significant (p < 0.05) preponderance of cleft palate among females and Moor ethnicity. Low birth weight and growth parameters were identified in preschool aged children. Ninety two (56.79%) had other clinical problems including four possible syndromes. The semi-quantitative PCR did not identify any subjects with a 22qll.2 deletion. The low rate of 22qll.2 deletion identified in this sample may be related to selection bias, non-exclusion of other syndromes or technical limitations but may also reflect a low prevalence of this syndrome in this cleft population. Further investigations are required to validate the semi-quantitative PCR method and generalize these findings to the Sri Lankan cleft palate population.
Evaluation of the clinical features and identification of 22q11 deletion among selected patients with cleft palate
(2013) Prabodha, L.B.L.
Incidence of pyramidalis muscle
(University of Kelaniya, 2011) Rajawasam, P.P.; Prabodha, L.B.L.; Gamage, U.; Nanayakkara, B.G.; Ilayperuma, I.; Hasan, R.
Pyramidalis is a triangular muscle that lies in front of the lower part of rectus abdominis muscle within the rectus sheath. This muscle is known to vary considerably in size and present either unilaterally or bilaterally. A large number of studies supports the racial variation in its occurrence. This preliminary study was carried out to investigate the incidence of pyramidalis muscle and also to establish its anatomical dimensions in a sample group of Sri Lankans. A total of forty two cadavers were studied during the routine gross anatomy dissections. The presence or absence of the muscle either bilaterally or unilaterally was noted on visual inspection. The maximum length and width of the pyramidalis was measured using a sliding caliper capable of measuring to the nearest 0.01mm. Pyramidalis was present bilaterally in 85.71% and absent in 14.28% of the subjects. The maximum length of the muscle was 6.24 ± 0.12 cm on right side and 7.09 ± 0.20 cm on left side. The maximum width was 1.32 ± 0.14 cm on right side and 1.52 ± 0.18 cm on left side. Bilateral variation in the dimensions of the muscle was statiscally significant. The occurrence of pyramidalis muscle was established for a group of adult Sri Lankan population. Our results reveal there is a racial trend in the incidence of pyramidalis muscle between Sri Lankan and other races such as black and white populations.
Molecular diagnosis of Velocardiofacial Syndrome in a cohort of Sri Lankan patients
(Sri Lanka Medical Association, 2014) Thevarajan, I.; Ranaweera, D.M.; de Silva, D.; Prabodha, L.B.L.; Gunasekera, R.; Dias, D.K.; Nanayakkara, B.G.; Basnayake, S.; Jayathilake, M.; Chandrasekharan, N.V.
INTRODUCTION AND OBJECTIVES: Velocardiofacial Syndrome (VCFS) is caused by a 3 Mb deletion encompassing around 40 genes on chromosome 22qll.2. It is characterised by variable features including congenital malformations of the palate and heart, growth and developmental delay, immunological anomalies, hypocalcaemia and other problems. Clinical diagnosis is difficult due to its variability within and between families. Early diagnosis enables appropriate management of the affected cases. Objective was to establish a reliable and cost effective molecular diagnostic test for VCFS. METHODS: Nineteen clinically suspected patients with palatal and facial features suggestive of VCFS from Lady Ridgeway Hospital, Colombo and the Teaching hospital, Karapitiya were recruited following informed consent and prior ethical clearance. A semi-quantitative multiplex poiymerase chain reaction (PCR) was established to identify the deletion using dosage analysis. The PCR assay was carried out using DNA from patients (P), unaffected person (N) and a positive control (with a FISH confirmed deletion} using STS markers within the deleted region and CFTR (Cystic Fibrosis Transmembrane Regulatory Conductance) control primers outside the deleted region. Following agarose gel electrophoresis the PCR products were quantified. A ratio of P: N of 0.5 was taken to indicate a deletion while a ratio of 1 indicated absence of the deletion. RESULTS: Among nineteen clinically suspected VCFS cases, five cases had the deletion. CONCLUSIONS: This semi-quantitative PCR assay was able to identify.deletions in clinically suspected patients. However further validation is required before its clinical usage.
Morphometrical analysis of the human palatine sutures
(University of Kelaniya, 2011) Udayanga, L.B.M.; Prabodha, L.B.L.; Palahepitiya, K.N.; Nanayakkara, B.G.; Ilayperuma, I.; Ambikaipakan, S.
The hard palate is traversed by a crucial suture which consists of mid palatal, interpalatine and transverse palatine sutures. The defects of the fusion of these palatine sutures lead to cleft palate. The goal of this study is to investigate the length and the gender differences of the palatine sutures in dried Sri Lankan skulls. A total of 63 (38 male; 25 female) dried human skulls were included in the study. The combined straight lengths of the midpalatal and interpalatine sutures and transverse palatine sutures were measured following the standard procedures with a spreading caliper capable of measuring to the nearest 0.01 mm. The mean combined lengths of the midpalatal and interpalatine sutures and transverse palatine sutures were (male: 46.76 ± 3.43; female: 44.96 ± 3.12); (male: 36.12 ± 4.6; female: 34.14 ± 2.9) respectively. Gender differences in the lengths of the palatine sutures were statistically significant (p<0.05). The results of this preliminary study establish the existence of a statistically significant sexual dimorphism in the lengths of the palatine sutures. Further, it provides a reference set of data on the lengths of the palatine sutures for an adult Sri Lankan population. Such data will facilitate the understanding of the etiopathogenesis of malformations of this cranial region and artificial teeth mould industry.