Browsing by Author "Prasadani, W.C."

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Comparison of the basic nutritional characteristics of the first extract and second extract of coconut milk
(2015) Nadeeshani, R.; Wijayaratna, U.N.; Prasadani, W.C.; Ekanayake, S.; Seneviratne, K.N.; Jayathilaka, N.
Determination of thermal stabilities of guava leaf, coconut cake, rice bran and sesame cake extracts
(Sri Lanka Association for the Advancement of Science, 2015) Senanayake, C.M.; Seneviratne, K.N.; Jayawardena, B.M.; Prasadani, W.C.
Effect of three edible oils on the intestinal absorption of caffeic acid: An in vivo and in vitro study.
(PLoS ONE 12(6), 2017) Prasadani, W.C.; Senanayake., C.M; Jayathilaka., N; Ekanayake., S; Seneviratne, K.N.
Polyphenolic antioxidants are mainly absorbed through passive paracellular permeation regulated by tight junctions. Some fatty acids are known to modulate tight junctions. Fatty acids resulting from the digestion of edible oils may improve the absorption of polyphenolic antioxidants. Therefore, we explored the effect of three edible oils on the intestinal absorption of caffeic acid. Rats were fed with soybean oil and caffeic acid dissolved in distilled water. Caffeic acid contents in the plasma collected up to 1 hr were quantified. The experiment was repeated with coconut oil and olive oil. Component fatty acids of the oils were individually tested in vitro for their effect on permeability of caffeic acid using Caco-2 cell monolayers. Highest absorption of caffeic acid was observed in animals fed with coconut oil. In vitro transport percentages of caffeic acid in 2.5 mmol/L solutions of fatty acids were 22.01±0.12 (lauric), 15.30 ± 0.25 (myristic acid), 13.59 ± 0.35 (linoleic acid), 3.70 ± 0.09 (oleic acid) and 0.10±2.0 (all other fatty acids). Lauric acid and myristic acid are the two major fatty acids present in coconut oil. Therefore, these fatty acids may contribute to the higher absorption of caffeic acid in the presence of coconut oil.
Efficacy of phenolic compounds of coconut cake on oxidative stability of pork
(Book of Abstracts, Annual Research Symposium 2014, 2014) Prasadani, W.C.; Seneviratne, N.A.K.P.J.; Jayawardena, B.M.
Antioxidant efficacy of different concentrations of phenolic compounds of coconut cake (PCCC) was determined using pork as a food model system. The extracts of PCCC were prepared using ethanol: water (70:30 v/v) and the antioxidant activity of the extracts on formation of thiobarbituric acid reactive substances (TBARS) was evaluated using a spectrophotometric method. Malondialdehyde, a secondary product of the oxidation of polyunsaturated fatty acids reacts with thiobarbituric acid and yield a pinkish red chromogen with an absorbance maximum at 532 nm. The effect of PCCC at 50, 100 and 200 ?g/g meat was compared with that of butylated hydroxy toluene (BHT) 200 ?g/ g and tert butylated hydroquinone (TBHQ) 200 ?g/ g. The extracts were effective in inhibiting the oxidation of pork in comparison with the control with no added phenolic extracts which showed the highest TBARS values during the storage period. At the end of day 5 of storage the inhibition percentage of TBARS are 26 � 9, 35� 7, 54� 4, 54� 4 and 56� 5 for TBHQ, BHT, PCCC 50, PCCC 100 and PCCC 200 respectively. PCCC were effective in suppressing the formation of TBARS in the employed system and their activity is higher than that of BHT and TBHQ. Coconut cake may be considered as a cheap potential source of phenolic compounds effective in stabilizing lipid foods.
Enzyme assisted extraction, quantification and antioxidant activity of phenolic compounds of coconut cake
(Sri Lanka Association for the Advancement of Science, 2013) Prasadani, W.C.; Seneviratne, K.N.; Jayawardena, B.M.
Improvement of the nutritional quality and shelf life of selected food systems by coconut based natural antioxidants
(2016) Prasadani, W.C.
Inhibition of lipid peroxidation of egg yolk homogenate by phenolic compounds in coconut cake
(Sri Lanka Association for the Advancement of Science, 2014) Prasadani, W.C.; Seneviratne, K.N.; Jayawardena, B.M.
Phenolic extracts of coconut oil cake: a potential alternative for synthetic antioxidants
(2016) Seneviratne, K.N.; Prasadani, W.C.; Jayawardena, B.
Limitations of natural antioxidants include relatively low antioxidant activity, narrow range of food systems where the antioxidants are effective and limited thermal stability compared to synthetic antioxidants. In the present study, the phenolic extract of coconut oil cake (COCE) was tested for antioxidant activity-related food stabilization. Heat stabilities of COCE and synthetic antioxidants were determined by measuring the induction time of sunflower oil enriched with heat-treated antioxidants. In the β-carotene-linoleate emulsion used for testing antioxidant activity, COCE can retain 96 ± 2% of initial colour intensity while BHT can retain 89 ± 2% of initial colour intensity at 60 μg mL–1 concentration after two hours. TBARS contents (MDA equivalents / kg of meat) in COCE-treated and control pork samples after 14 days was 2.80 ± 0.57 and 22.55 ± 2.30 respectively. Heat stability varies in the order butylated hydroxytoluene (BHT) < (butylated hydroxyanisole (BHA) < COCE < tertiary butylhydroxyquinone (TBHQ). The results of these experiments suggest that COCE is a versatile and thermally stable natural antioxidant mixture effective in stabilizing many food systems.
Preliminary investigation of polycyclic aromatic hydrocarbon levels in smoked fish produced by fisher community in Sri Lanka
(Sri Lanka Association for Fisheries and Aquatic Resources, 2016) Dehikumbura, G.A.M.D.K.; Ganegama Arachchi, G.J.; Senevirathne, K.N.; Prasadani, W.C.; Paththuwe Arachchi, M.; Perera, R.; Wickramasinghe, I.
Subcritical water extraction of phenolic compounds from coconut cake
(Sri Lanka Association for the Advancement of Science, 2015) Prasadani, W.C.; Seneviratne, K.N.; Jayawardena, B.M.
Thermal stability of phenolic compounds in coconut cake and its stabilizing effect on stripped sunflower oil
(Faculty of Graduate Studies, University of Kelaniya, 2015) Prasadani, W.C.; Jayawardena, B.M.; Seneviratne, K.N.
Coconut cake possesses phenolic compounds which are antioxidatively active in chemical and food model systems. However, the thermal stability of these phenolic compounds has not yet been investigated. In this study, the thermal stability of phenolic compounds in coconut cake (PCCC) was compared with that of synthetic antioxidants, butylated hydroxy toluene (BHT), butylated hydroxy anisole (BHA) and tert butyl hydroxy quinone (TBHQ) using two food model systems. PCCC were extracted using ethanol:water (70:30 v/v) and the phenolic concentration was determined using the Folin-Dennis method. Thermal stability was tested by heating PCCC and other synthetic antioxidants at 180 oC up to two hours. In 30 min intervals, the activity of heated antioxidants were tested by evaluating their ability to inhibit thiobarbituric acid reactive substances (TBARS) formation in egg yolk emulsion (EYEM). The percentage inhibition of TBARS formation was calculated against a control EYEM sample prepared without added antioxidants. Protective effect of antioxidants on stripped sunflower oil (SSO) was also evaluated. For this purpose, PCCC and synthetic antioxidants were heat treated at 180 oC for two hours and these antioxidants were incorporated into SSO. The induction time (IT) of SSO was determined at 100 oC in the Rancimat apparatus. The percentage inhibition of TBARS formation in EYEM by BHT, BHA, TBHQ and PCCC decreased with heating time and the percentage inhibition of all antioxidants decreased below 40 % after two hours of heating at 180 C. However, at 30 min of heating, inhibition percentage of TBARS formation by PCCC (72±4 %) and TBHQ (68±2 %) is considerably higher compared to BHT (54±2 %) and BHA (42±2%). The IT of SSO varied in the order, control (1.85±0.14 h) < BHT (2.06±0.08 h) < BHA (2.14±0.06 h) < PCCC (2.18±0.03 h) < TBHQ (2.44±0.10 h). The results of these experiments suggest that PCCC can be used to stabilize foods under high temperature cooking conditions.