Browsing by Author "Seran, T.H."

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Agronomic evaluation of brinjal (Solanum melongena L.) and groundnut (Arachis hypogaea L.) intercropping system
(Gampaha Wickramarachchi Ayurveda Institute, 2009) Prashaanth, R.; Seran, T.H.; Brintha, I.; Sivachandiran, S.
Callus induction in anther culture of selected Capsicum annuum L. varieties
(Research Symposium on Pure and Applied Sciences, 2018 Faculty of Science, University of Kelaniya, Sri Lanka, 2018) Rajakaruna, R.H.M.S.V.; Kumari, H.M.P.S.; Seran, T.H.
Plant breeding programs of capsicum can be accelerated through double haploid plant production. One of the easier ways to produce double haploid plants is anther culture. This experiment was carried out to induce anther callus of capsicum (Capsicum annuum L.) breeding line 1782. For comparison of the behavior of breeding line 1782 in callus induction, Lanka Yellow Wax (LYW), a recommended variety of capsicum was used. Unopened flower buds were collected from the above selected varieties and anthers were excised. Anthers at late uninucleate stage were sterilized and cultured on four different MS media supplemented with 2, 4-D and BA (1 mg/L 2, 4-D with 2 mg/L BA (medium 1), 2 mg/L 2, 4-D with 2 mg/L BA (medium 2), 2 mg/L 2, 4-D with 2.5 mg/L BA (medium 3) and 2 mg/L 2, 4-D with 3 mg/L BA (medium 4) to induce formation of callus. The cultured anthers were incubated in dark for 14 days at 250C. After the callus induction, selected calli were transferred into a regeneration MS medium with 5 mg/L BA and 1 mg/L IAA. Regenerating cultures were incubated in 16 hr light conditions at 250C and observations were recorded for appearance and greening of calli. The results revealed that medium 1 and also medium 3 had significant effect on anther callus induction than other media. In medium 3, the time taken for callus induction was minimum and also the callus induction percentage was higher than other callus induction media. Although, medium 1 and 3 performed in the same manner for callus induction for both breeding line 1782 and LYW, the highest performance was shown by medium 3. In 1782 breeding line and LYW variety comparison, it was found that LYW variety was better than 1782 breeding line and had a significant (P<0.05) effect on anther callus induction. The callus induction percentage of 1782 breeding line was 59.6% and it was lower than LYW (83.9%). Anther calli formed in medium 3 showed best performance in regeneration medium and calli of breeding line 1782 performed better than LYW variety. In the regeneration medium, calli behaved in different ways. White crystalline calli responded well to the regeneration medium and calli enlargement and, greening at later stage was observed. Light brown coloured calli did not show enlargement and greening compared to crystal calli. The anther calli generation protocol developed in this study could be used to regenerate haploid plants of selected capsicum varieties.
Embryogenic response of aerial stem of ginger (Zingiber officinale Rosc.) cultured in different media
(University of Kelaniya, 2011) Sathyagowri, S.; Seran, T.H.
Ginger (Zingiber officinale Rosc.) is a herbaceous perennial which is valued as an important spice and medicinal crop throughout the world, and it is vegetatively propagated through rhizome buds. This study was done to find out the optimal hormonal combination for the initiation of somatic embryos in aerial stem explants of ginger (Zingiber officinale Rosc.). The aerial stems at the basal portion were collected from the field grown local variety of ginger and rinsed thoroughly in running water followed by distilled water and then dipped in 70% ethanol for 1 min. Subsequently, surface sterilization was done using 20% of CloroxTM prior to culture on MS medium. The sterilized explants (1 cm long) were vertically excised into two halves and cultured on MS medium with two different BAP concentrations with or without 0.1 mg/l NAA or IAA. The results showed that initial swelling and greening of explants exhibited during first week of culture and the size of the explants increased two third folds after four weeks in culture containing BAP alone, while others showed a low degree of swelling. The size increase may be due to active cell division and expansion resulted by exogenous supply of growth regulator. Further, it was noted that the explants cultured in medium solely contained BAP only produced whitish yellow globular structures which have the potential to become somatic embryos. The degree of embryogenic response was increased with the increase in BAP concentration from 1 mg/l to 2 mg/l. The BAP in combination with NAA or IAA led the explants to initiate shoot buds and roots which led to produce plantlets via organogenesis. Moreover, histological study revealed the origin and development of somatic embryos directly from the aerial stem explants without intervening callus stage. The meristamatic cells of the explants further differentiated into embryogenic cells. Continuous division of these cells produced the globular mass of proembryoids and differentiation of these structures led to the formation of somatic embryos for mass propagation of ginger under in vitro conditions.