Browsing by Author "Singhalage, I. D."

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Effect of sugars, amino acids, hormones and microbial biofilm exudates on dormancy breaking of culturable soil microbial seed bank.
(International Research Symposium on Pure and Applied Sciences, 2017 Faculty of Science, University of Kelaniya, Sri Lanka., 2017) Wijepala, P. C.; Jayasena, P. G.; Singhalage, I. D.; Seneviratne, G.; Madawala, H. M. S. P.; Jayasuriya, K. M. G. G.
Soil microbial diversity is found to be beneficial for plant growth and productivity. The diversity has decreased to alarming level under stressful soil conditions and microbes form dormant structures like spores to withstand stressful soil conditions. Biofilm Biofertilizer (BFBF) is the most efficient product, newly introduced to the agricultural industry of Sri Lanka, which consists of different compounds like sugars, amino acids and hormones. These compounds assist in breaking the dormancy of microbial seed bank. However, effect of the compounds on the dormancy breaking has been poorly investigated. Therefore in the current study, the effect of glucose, tryphtophan, indole acetic acid (IAA), BFBF exudates on dormancy breaking and growing of culturable soil microbial seed bank was investigated. BFBF exudates and seven types of solutions were prepared by mixing glucose, tryptophan and IAA in different ratios and used them as treatments. Sterile distilled water was used in the control. Sieved (0.5 mm) and air dried garden soil was treated and incubated for 2 months under room temperature. Triplicated treatments were arranged according to completely randomized design. Then the dormancy breaking test was conducted on treated soil following the protocols developed by National Institute of Fundamental Studies. Bacterial colony count and average colony diameters under each treatment were recorded after 1, 2, 4 and 6 weeks. Fourier Transformed Infrared (FTIR) spectra were recorded for exudates and prepared solutions. The highest colony count was observed in BFBF exudate treatment throughout the experiment whereas highest diameter was different among treatments during the incubation period. The lowest colony count was recorded in soil treated by IAA. This implies that the bacterial growth was improved when compounds interacted. The study concludes that colony count and diameter varies among treatments. However, among all treatments, BFBF exudate was the best for microbial seed bank dormancy breaking.
The vegetation structure and floristic composition of tropical rainforest landscape, at Udakiruwa, Sri Lanka
(Faculty of Science, University of Kelaniya Sri Lanka, 2023) Sanjeewa, U. D. L.; Singhalage, I. D.; Perera, G. A. D.; Kaliyadasa, P. E.; Kumara, P. H. T.; Ediriweera, E. P. S. K.
The Udakiruwa rainforest fragments in the Uva province, Sri Lanka, represent the last remaining refugia in the Eastern part of the country. Within these forest fragments, populations of endemic plant species thrive, however, many of them are now threatened due to overexploitation of forest products and agricultural expansions. To address the conservation of these endangered species and the maintaining of ecosystem services in Udakiruwa forest fragments, baseline information is crucial. Therefore, this study aimed to examine the species composition and structure of the forests in both old-growth and secondary forest landscapes in Udakiruwa. In this study, we recorded the diameter and species composition of trees in 44 randomly located circular plots, where the tree diameter at breast height (DBH) was greater than 5 cm. The individuals were categorized into five DBH classes (5-9.9 cm, 10-29.9 cm, 30-49.9 cm, 50-69.9 cm, and >70 cm). A total of 1408 individuals, representing 79 species, 38 families, and 62 genera, were recorded from this comprehensive study. Out of the total number of species identified, 25 were endemic. Mallotus fuscescens and Dipterocarpus zeylanicus emerged as the most abundant species as 70% of the established experimental plots. However, the endemic species such as Shorea dyeri (5%), Euonymus walkeri (0.1%), Strombosia ceylanica (2%), Palaquium hinmolpedda (1%), Diospyros ebenoides (0.1%), Mangifera zeylanica (3%), Calophyllum tomentosum (0.1%), and Garcinia quaesita (1%) were found to be present in very low numbers. The total basal area of individuals in the site was measured to be 912.615 m2, and further investigation revealed that 28 out of 44 plots had experienced selective logging. Notably, 87.5% of recorded Shorea dyeri in the experimental plots fell under the DBH classes of 5-9.9 cm and 10-29.9 cm, while Dipterocarpus zeylanicus was most abundant in the DBH size class >70 cm. The calculated Shannon diversity index and evenness of species were 1.823 and 0.780, respectively, indicating a diverse and relatively balanced ecosystem. However, sites closer to rubber cultivations and those located at the forest edge reported a high disturbance index (7.879), suggesting the impact of human activities on these fragmented lowland forests. The lowland fragmented forest landscapes of Udakiruwa are rich with many endemic species, emphasizing the urgent need for immediate conservation actions to conserve this unique rainforest ecosystem.