Browsing by Author "Sudesh, A.D.H."

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An analytical study on the antimicrobial activity of Visarpahara Tailaya against Candida albicans and Staphylococcus aureus.
(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Madumali, M.G.B.; Wickramarachchi, W.J.; Karunarathna, E.D.C.; Sudesh, A.D.H.
Visarpahara Tailaya is a herbo-mineral preparation recommended to use clinically in the management of eczema, scabies, itches and other types of skin diseases mainly caused by bacteria and fungi. This study was conducted to determine the antimicrobial activity of this drug using the standard Antimicrobial Sensitivity Test against Staphylococcus aureus and Candida albicans. The test was performed according to the Well diffusion Method having 6 mm diameter wells on Mueller Hinton Agar (MHA) for S. aureus and Sabouraud Dextrose Agar (SDA) for C. albicans. As the positive controls, Amoxicillin 10mg/ml was used for S.aureus and Fluconazole 2.5mg /ml for C. albicans. According to the results, the test drug showed an average Inhibition Zone Diameter (IZD) of 12mm for S. aureus and 17mm for C. albicans while the positive controls showed 51mm and 25mm respectively .These results show that the test drug Visarpahara Thailaya is effective for both test organisms with an increased effectiveness for C.albicans compared to S. aureus. Therefore, it is concluded that Visarpahara Tailaya can be effectively used in the management of C. albicans while it is less effective for S. aureus.
Antibacterial activity of extract of leaves of Atalantia ceylanica (Yakinaran)
(Faculty of Graduate Studies, University of Kelaniya, 2015) Munasinghe, D.A.L.; Karunarathna, E.D.C.; Sudesh, A.D.H.
Antibacterial activity of leaves of Atalantia ceylanica was tested qualitatively against 05 bacterial strains with the modified plate method at Gampaha Wickramarachchi Ayurveda Institute. The steam of boiled leaves and extract are used by certain villagers as a traditional treatment for healing respiratory ailments such as influenza. Even though natural flora may be effective in curing health defects, scientific studies on subject is limited. Thus the study was arranged to fill the gap for certain extent to harness natural resources effectively in health. So as the study was aimed to investigate the antibacterial properties of Atlanta leaves. The plates, prepared with the watery extract of boiled leaves, which comprised of neat concentration of raw leaf materials and Mueller-Hinton agar were inoculated with laboratory stored standard bacterial strains including Pseudomonas aeruginosa, Streptococcus pneumonia, Escherichia coli, Staphylococcus aureus and Methicillin resistant Staphylococcus aureus separately, which can cause respiratory as well as gastro- intestinal symptoms. For the control test, distilled water was used to make plates instead of watery leaf extract. Even after the duplicated experiment, no strain was found to be inhibited by the leaf materials, which indicated that the neat concentration of raw chemical compound of particular leaves have no inhibitory action on tested strains. As the respiratory infection is not only caused by bacteria but also by viruses, perhaps phytochemicals might have possessed antiviral as well as antibacterial properties on organisms other than the tested strains. Occasionally the chemicals of the particular plant leaves might have possessed certain antiinflammatory features to control respiratory symptoms. Nevertheless it was clear that the chemical compounds of the distillation of leaves of Atalantia ceylanica had no significant inhibitory effect on the growth of tested strains. The same experiment was carried out early for the steam of particular leaf and the results were found to be the same.
Antibacterial activity of two different Prathisaranas against periodontitis.
(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Abegunasekara, N.S.; Peiris, K.P.P.; Sudesh, A.D.H.
'Kushtaadi Choorna and Shwethamanjana Choorna' are used in the treatment ofDanthamulagatha Roga such as Sheethada, Danthaweshta and Upakusha. These formulations are used specially as a local treatment. Staphylococcus aureus and Methicillin resistant Staphylococcus aureus (MRSA) are main causative organisms of periodontal diseases. The present study was carried out to determine the antibacterial activity of Kushtaadi choorna andShwethamanjana choorna using agar well diffusion method in comparison with standard antibiotic Amoxicillin against the Staphylococcus aureus (ATCC 25923) and Methicillin resistant Staphylococcus aureus (MRSA). The samples of Prathisarana were prepared in ne powder form, Amoxicillin as positive control and triplicates were made for each test. Observation was recorded by measuring the diameter of the inhibitory zones surrounding the wells. According to the results, inhibitory effect of both Prathisarana samples were signicantfor Staphylococcus aureus and Methicillin resistant Staphylococcus aureus (MRSA).
A comparative study on the anti-microbial efficacy of two Ayurveda powders (Prathisarana) used in periodontal diseases
(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Wijebandara, D.M.K.K.; Peiris, K.P.P.; Karunarathna, E.D.C.; Sudesh, A.D.H.
Kushtadi (KU) and Karanjadi (KR) are prominently used Ayurvedic powders (Prathisarana) in the management of periodontal diseases. This study was carried out for the determination of antimicrobial efcacy of these two drugs using standard Antimicrobial Sensitivity Test against Candida albicans and a fungal culture isolated from a patient. The Antimicrobial Sensitivity Test was performed according to the Well diffusion method having 6mm diameter wells on Sabouraud Dextrose Agar (SDA). Each well was loaded with test drugs mixed in 20μl of sterile distilled water and uconazole 2.5mg / ml as the positive control. According to the results, KR showed an average Inhibition Zone Diameter (IZD) of 20mm for the isolated culture while KU did not show any inhibition. The results were similar for Candida albicans, KR showing IZD 18 mm, KU IZD 0 mm while positive control showed an inhibition of 25 mm. Therefore, it can be concluded that both Candida albicans and the isolated culture were sensitive only for KR making it the only effective drug out of the two drugs tested in this study.
Determination of antimicrobial efficacv of four Ayurveda Local Applications (Pratisarana Yoga) in the management of Periodontal Disease
(International Journal of Applied Science and Research, Volume 2, Issue 3, 2019) Peiris, K.P.P.; Wanigasekara, D.N.; Sudesh, A.D.H.; Katunatathne, E.D.C.
This study evaluates the antimicrobial activity of four Ayurvedic formulations namely Kushtadi Ehurna, Swethamaniana Churna, Karanladi Churna and Dasana Sanskara Churna that are used to treat periodontal diseases as local applications. The objectives are to find out the presence or absence of in-vitro antimicrobial efficacy of these four drugs and to evaluate the antimicrobial efficacy of them using agar well diffusion method. The organisms used for the study are Staphylococcus aureus (ATCC 29213), Metlucillin resistant Staphylococcus aureus (ATCC 25923) and clinically isolated Candida albicans. The results of the in-vitro antimicrobial efficacy study reveals that Swethamanjana Chuma possesses antimicrobial action against all three tested microorganisms. Kushtadi Churna is cffcctivc against S. aureus (ATCC 29213) and MRSA (ATCC 25923) and it is unable ro affect rhe growth of C. albicans. Karaniadi Churna and Dasana Sanskara Chuma are effecnve against S. aureus (ATCC 29213) and C. albicans and not effective against MRSA(ATCC 25923). Fluconazole and Amoxicillin are used as positive controls for the yeast and two bacteria respectively.
Determination of antimicrobial efcacy of Triphala Ashchyotana as a therapeutic agent for acute conjunctivitis.
(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Perera, B.S.R.; Dayarathna, M.T.A.; Perera, B.P.R.; Karunarathne, E.D.C.; Sudesh, A.D.H.
Triphala decoction is a therapeutic agent used in the form of Ashchyotana (eye drops) for acute conjunctivitis. The antibacterial assay was performed according to the standard Antibiotic Sensitivity Test (ABST) for Staphylococcus aureus (ATCC 25923), Escherichia coli, Pseudomonas aeruginosa, Methicillin Resistant Staphylococcus aureus (MRSA) and Candida albicans using chloramphenicol and uconazole as positive controls. The test drug, Triphala decoction, was prepared by boiling dry samples of Terminalia chebula, Terminalia belerica and Phyllanthus emblica 20g each, in 1920ml of water and reducing it to 240ml. Based on the results of ABST, the Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) against S. aureus was determined by using a concentration series ranging from 3 X 50 μl to12 X 50 μl of Triphala decoction with 100μl of S. aureus in 10ml of nutrient broth. The volumes were equalized up to 11ml using sterilized distilled water. After an overnight, incubation at 37OC, 500μl from each concentration was plated using pour-plate technique and incubated overnight at 37OC. To determine the MLC, a loopful of each concentration was streaked on Nutrient Agar plates and incubated overnight at 37OC. According to the results, S. aureus was sensitive to Triphala decoction making an inhibition zone diameter (IZD) of 18mm, while the readings for the other species were in the intermediate range of 17mm for E.coli and 13mm for P. aeruginosa. MRSA strain was resistant to the test drug making IZD 11mm and no inhibition zone was observed for Candida albicans. The MIC and MLC for S. aureus were of the same concentration (6 X 50μl) showing zero growth after that point. It can be concluded that Triphala ashchotana is effective in controlling acute conjunctivitis caused by S. aureus and the dosage required is 6 X 50 μl (6 drops). Triphala can also be used as a prophylactic agent against acute conjunctivitis.
In-vitro evaluation of antimicrobial efficacy of the indigenous formulation, Karna Bindu against common pathogens causing otitis infections.
(International Journal of Scientific Research and Innovative Technology, Volume 6, Issue 7, 2019) peiris, K.P.P.; Wanigasekara, D.N.; Sudesh, A.D.H.; Karunarathne, E.D.C.
This study investigates the efficacy of the formulation named "Karna Bindlö' which has been used in indigenous medicinal practices to treat ear infections such as otitis media and otitis externa. The objectives are to determine the antimicrobial efficacy of Karna Bindu against Aspergillus niger,Candida alhicans and Pseudomonas aeruginosa and to compare the efficacy of the indigenous formulation with the conventional antibiotics that are used for the test organisms. By using the agar well diffusion method, the test organisms were cultivated and the relevant antibiotics were used as positive controls while sterile distilled water served as the negative control. The antimicrobial assay revealed that Karna Bindu is effective against all three selected microorganisms. The Minimum Inhibitory Concentrations (MIC) of the formulation for C. albicans and aeruginosa were recorded as 500 gL/mL, and 750 gL/mL accordingly.
Microbiological study of an indigenous mouth wash (Gandusha) used in periodontal diseases.
(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Dewasurendra, D.M.W.; Peiris, K.P.P.; Karunarathna, E.D.C.; Sudesh, A.D.H.
Mouth wash (Gandusha) is clinically effective in the management of periodontal diseases. This study is an in-vitro analysis on determining the antimicrobial efcacy of this drug using standard Antimicrobial Sensitivity Test against a fungal culture isolated from a patient. The test was performed according to the Well diffusion method having 6mm diameter wells on Sabouraud Dextrose Agar (SDA). Each well was loaded with the test drug mixed in 20μl of sterile distilled water and uconazole 2.5mg / ml was used as the positive control. According to the results, the test drug showed an average Inhibition Zone Diameter (IZD) of 19mm while the positive control showed IZD of 15mm. Therefore, it can be stated that the test drug is more effective than the positive control and the isolated culture is sensitive to the test drug as it had shown an IZD of 19mm while it is intermediate sensitive for the positive control as 15mm comes under Intermediate sensitive range (15-18mm). These results support the claim that the indigenous mouth wash (Gandusha) is effective in the management of periodontal diseases.