YARIS - 2019
Permanent URI for this collectionhttp://repository.kln.ac.lk/handle/123456789/20839
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Item Evaluation of the antibacterial effect of Acronychia pedunculata fresh extract against Staphylococcus aureus: a study in vitro(Young Ayurveda Researchers' and Innovators' Symposium (YARIS – 2019), Institutional Research Committee, Gampaha Wickramarachchi Ayurveda Institute, University of Kelaniya, Yakkala, Sri Lanka, 2019) Muthukuda, M.A.C.LIn vitro antibacterial activity of fresh extracts of stem bark, leaves and combined sample of Acronychia pedunculata found in Sri Lanka were investigated against Staphylococcus aureus (ATCC 25923) and found the Minimum Inhibitory Concentration. Kirby Baurer Well diffusion method of antibacterial Susceptibility Testing was used to determine antibacterial activity of each fresh extract. Dilution series for Minimum Inhibitory Concentration were prepared. Amoxacillin and distilled water were used as positive and negative control respectively. Zone diameters were interpreted and data were statistically analyzed. The results showed that there was antibacterial effect of each sample. The highest zone diameter was observed for the combined sample; fresh extracts of stem bark and leaves (11.67mm). This could be due to the synergetic activity of each compound from each plant material used in this study. Fresh extracts of stem bark showed the second most (11mm) and fresh extracts of leaves the least (9mm). As fresh extracts of stem bark and leaves of Acronychia pedunculata (combined sample) showed the highest activity, dilution series from that sample were prepared. The minimum inhibitory concentration of combined sample for the growth of Staphylococcus aureus was 10-2 mg/ml. This could be further investigated for more gram positive and negative bacterial and fungal species. It is worth to investigate each extract individually using various chromatographic techniques and identify the active ingredients separately.Item Evaluation of the anti-bacterial effect of traditional Lodhradi lepa and Lodhradi cream against the laboratory specimen of staphylococcus aureus: a laboratory experiment(Young Ayurveda Researchers' and Innovators' Symposium (YARIS – 2019), Institutional Research Committee, Gampaha Wickramarachchi Ayurveda Institute, University of Kelaniya, Yakkala, Sri Lanka, 2019) Madushani, P.A.K.; Wickramarachchi, W.J.Acne vulgaris is a chronic disease which destroys the beauty and cause disfigurement of the face especially in the adolescence. Acne vulgaris is known as Mukhadushika or Yuvana Pidaka in Ayurveda. Acne vulgaris, secondary infected by Staphylococcus aureus. Lodhradi lepa is an external application for Yuvana Pidaka in Ayurveda. The study was to test the anti-bacterial activity of the traditional Lodhradi lepa and Lodhradi cream against the Staphylococcus aureus. The anti-bacterial sensitivity test was done on well diffusion method with freshly prepared drugs. In trial one it was tested with freshly prepared drugs and the second trial was done with two different concentrations of freshly prepared drugs. In both trials the zone diameters were among 00-12mm and as the diameters were not among the resistant or susceptible level Staphylococcus aureus is not susceptible to these external ointments. Erythromycin the positive one was highly susceptible. Staphylococcus aureus was not susceptible for the traditional Lodhradi lepa and Lodhradi cream. The traditional Lodhradi lepa and Lodhradi cream would be used in acne vulgaris to reduce inflammation like symptoms due to its pacifying properties.