1st-2017
Permanent URI for this collectionhttp://repository.kln.ac.lk/handle/123456789/17673
Browse
5 results
Search Results
Item Determination of antimicrobial efcacy of Triphala Ashchyotana as a therapeutic agent for acute conjunctivitis.(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Perera, B.S.R.; Dayarathna, M.T.A.; Perera, B.P.R.; Karunarathne, E.D.C.; Sudesh, A.D.H.Triphala decoction is a therapeutic agent used in the form of Ashchyotana (eye drops) for acute conjunctivitis. The antibacterial assay was performed according to the standard Antibiotic Sensitivity Test (ABST) for Staphylococcus aureus (ATCC 25923), Escherichia coli, Pseudomonas aeruginosa, Methicillin Resistant Staphylococcus aureus (MRSA) and Candida albicans using chloramphenicol and uconazole as positive controls. The test drug, Triphala decoction, was prepared by boiling dry samples of Terminalia chebula, Terminalia belerica and Phyllanthus emblica 20g each, in 1920ml of water and reducing it to 240ml. Based on the results of ABST, the Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) against S. aureus was determined by using a concentration series ranging from 3 X 50 μl to12 X 50 μl of Triphala decoction with 100μl of S. aureus in 10ml of nutrient broth. The volumes were equalized up to 11ml using sterilized distilled water. After an overnight, incubation at 37OC, 500μl from each concentration was plated using pour-plate technique and incubated overnight at 37OC. To determine the MLC, a loopful of each concentration was streaked on Nutrient Agar plates and incubated overnight at 37OC. According to the results, S. aureus was sensitive to Triphala decoction making an inhibition zone diameter (IZD) of 18mm, while the readings for the other species were in the intermediate range of 17mm for E.coli and 13mm for P. aeruginosa. MRSA strain was resistant to the test drug making IZD 11mm and no inhibition zone was observed for Candida albicans. The MIC and MLC for S. aureus were of the same concentration (6 X 50μl) showing zero growth after that point. It can be concluded that Triphala ashchotana is effective in controlling acute conjunctivitis caused by S. aureus and the dosage required is 6 X 50 μl (6 drops). Triphala can also be used as a prophylactic agent against acute conjunctivitis.Item Antibacterial activity of two different Prathisaranas against periodontitis.(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Abegunasekara, N.S.; Peiris, K.P.P.; Sudesh, A.D.H.'Kushtaadi Choorna and Shwethamanjana Choorna' are used in the treatment ofDanthamulagatha Roga such as Sheethada, Danthaweshta and Upakusha. These formulations are used specially as a local treatment. Staphylococcus aureus and Methicillin resistant Staphylococcus aureus (MRSA) are main causative organisms of periodontal diseases. The present study was carried out to determine the antibacterial activity of Kushtaadi choorna andShwethamanjana choorna using agar well diffusion method in comparison with standard antibiotic Amoxicillin against the Staphylococcus aureus (ATCC 25923) and Methicillin resistant Staphylococcus aureus (MRSA). The samples of Prathisarana were prepared in ne powder form, Amoxicillin as positive control and triplicates were made for each test. Observation was recorded by measuring the diameter of the inhibitory zones surrounding the wells. According to the results, inhibitory effect of both Prathisarana samples were signicantfor Staphylococcus aureus and Methicillin resistant Staphylococcus aureus (MRSA).Item A comparative study on the anti-microbial efficacy of two Ayurveda powders (Prathisarana) used in periodontal diseases(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Wijebandara, D.M.K.K.; Peiris, K.P.P.; Karunarathna, E.D.C.; Sudesh, A.D.H.Kushtadi (KU) and Karanjadi (KR) are prominently used Ayurvedic powders (Prathisarana) in the management of periodontal diseases. This study was carried out for the determination of antimicrobial efcacy of these two drugs using standard Antimicrobial Sensitivity Test against Candida albicans and a fungal culture isolated from a patient. The Antimicrobial Sensitivity Test was performed according to the Well diffusion method having 6mm diameter wells on Sabouraud Dextrose Agar (SDA). Each well was loaded with test drugs mixed in 20μl of sterile distilled water and uconazole 2.5mg / ml as the positive control. According to the results, KR showed an average Inhibition Zone Diameter (IZD) of 20mm for the isolated culture while KU did not show any inhibition. The results were similar for Candida albicans, KR showing IZD 18 mm, KU IZD 0 mm while positive control showed an inhibition of 25 mm. Therefore, it can be concluded that both Candida albicans and the isolated culture were sensitive only for KR making it the only effective drug out of the two drugs tested in this study.Item Microbiological study of an indigenous mouth wash (Gandusha) used in periodontal diseases.(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Dewasurendra, D.M.W.; Peiris, K.P.P.; Karunarathna, E.D.C.; Sudesh, A.D.H.Mouth wash (Gandusha) is clinically effective in the management of periodontal diseases. This study is an in-vitro analysis on determining the antimicrobial efcacy of this drug using standard Antimicrobial Sensitivity Test against a fungal culture isolated from a patient. The test was performed according to the Well diffusion method having 6mm diameter wells on Sabouraud Dextrose Agar (SDA). Each well was loaded with the test drug mixed in 20μl of sterile distilled water and uconazole 2.5mg / ml was used as the positive control. According to the results, the test drug showed an average Inhibition Zone Diameter (IZD) of 19mm while the positive control showed IZD of 15mm. Therefore, it can be stated that the test drug is more effective than the positive control and the isolated culture is sensitive to the test drug as it had shown an IZD of 19mm while it is intermediate sensitive for the positive control as 15mm comes under Intermediate sensitive range (15-18mm). These results support the claim that the indigenous mouth wash (Gandusha) is effective in the management of periodontal diseases.Item An analytical study on the antimicrobial activity of Visarpahara Tailaya against Candida albicans and Staphylococcus aureus.(Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Madumali, M.G.B.; Wickramarachchi, W.J.; Karunarathna, E.D.C.; Sudesh, A.D.H.Visarpahara Tailaya is a herbo-mineral preparation recommended to use clinically in the management of eczema, scabies, itches and other types of skin diseases mainly caused by bacteria and fungi. This study was conducted to determine the antimicrobial activity of this drug using the standard Antimicrobial Sensitivity Test against Staphylococcus aureus and Candida albicans. The test was performed according to the Well diffusion Method having 6 mm diameter wells on Mueller Hinton Agar (MHA) for S. aureus and Sabouraud Dextrose Agar (SDA) for C. albicans. As the positive controls, Amoxicillin 10mg/ml was used for S.aureus and Fluconazole 2.5mg /ml for C. albicans. According to the results, the test drug showed an average Inhibition Zone Diameter (IZD) of 12mm for S. aureus and 17mm for C. albicans while the positive controls showed 51mm and 25mm respectively .These results show that the test drug Visarpahara Thailaya is effective for both test organisms with an increased effectiveness for C.albicans compared to S. aureus. Therefore, it is concluded that Visarpahara Tailaya can be effectively used in the management of C. albicans while it is less effective for S. aureus.