Volume 09 - 2014
Permanent URI for this collectionhttp://repository.kln.ac.lk/handle/123456789/13906
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Item Development of Suitable Propagation Systems for Tephrosia spp. in Sri Lanka(University of Kelaniya, 2014) Priyadarshan, A.I.S.; Senanayake, S.P.; Jayatilleke, M.P.; Rajapakse, S.Genus Tephrosia (Fabaceae) commonly known as ‘Pila’ in Sri Lanka. Some Tephrosia spp. are used in many traditional therapeutic preparations in Sri Lanka and also in other countries. Increased use of Tephrosia in traditional therapeutic systems has developed a great demand for these species. Thus production of planting materials for commercial cultivation has become a timely requirement. Therefore, this research was conducted to determine suitable propagation systems for four selected Tephrosia spp.; T. purpurea, T. villosa, T. noctiflora and T. pumila. Even though seeds of Tephrosia spp. showed high viability, lower germination percentage was observed on wet filter paper as well as in six different potting media [M0 (Coir: dust: Sand; 1:1:1), M1 (Coir dust: Sand: Top soil; 1:1:1), M2 (Coir dust: Sand: Top soil: Compost; 1:1:1:1), M3 (Coir dust: Sand: Top soil: Compost: Cow dung; 1:1:1:1:1), M4 (Coir dust: Sand: Compost; 1:1:1), M5 (Coir dust: Sand: Top soil: Cow dung; 1:1:1:1)] during a period of one week. Water impermeability of seed coats was identified as the main reason for the seed dormancy in Tephrosia spp. and soaking seeds in Con. H2SO4: H2O; 3:1 for 25 minutes and rubbing seeds on sandpaper for 2 minutes were determined as suitable dormancy breaking methods. Variation was observed in the growth performance of Tephrosia spp. in six different potting media. Growth performances of seedlings of Tephrosia spp. in hydroponic solution and soil medium were observed and soil medium was selected as the most suitable medium for the growth of Tephrosia species based on their growth performances.Item Intraspecific Variation of Flueggea leucopyrus Willd. Grown in Sri Lanka and Establishing a Suitable Propagation System(University of Kelaniya, 2014) Priyadarshan, A.I.S.; Senanayake, S.P.; Jayatilleke, M.P.; Rajapakse, S.Flueggea leucopyrus belongs to family Phyllanthaceae, commonly known as ‘Katupila’or ‘Heen katupila’ and has become increasingly popular as an ingredient in several traditional therapeutic systems in Sri Lanka as well as in other Asian countries. A questionnaire survey was carried out using a hundred population sample to determine the awareness of the distribution and therapeutic uses of F. leucopyrus in Sri Lanka as a preliminary survey. The aims of the present study were to interpret the phenetic variation and determine the variation of phenolic composition in leaf extracts of F. leucopyrus in different climatic zones in Sri Lanka and to develop a suitable propagation system for F. leucopyrus. Eleven floral and vegetative characters of F. leucopyrus were observed and cluster analysis was carried out to infer phenetic relationships among populations collected from localities, Colombo, Kurunegala, Puttalam and Hambanthota, .representing different climatic zones. Medicinal value of most of plants lies in phytochemicals such as phenolics, flavonoids and anthocyanidins. Thus, leaf samples were extracted into ether, and ether extracts were used to isolate phenolic compounds using 2-Dimensional paper chromatography in solvent systems, butanol: acetic acid: water; 6:7:3 and sodium formate: formic acid: water; 10:1:200. Colors of the spots were observed under UV (366nm), and Rf values were calculated. Intra specific variations were observed in the distribution of phenolics in leaves and phenetic characters of F. leucopyrus with respect to their habitats in different climatic zones. Due to low seed production in F. leucopyrus, stem cuttings (Semi-hardwood, hardwood and softwood) in three different potting media were used to evaluate the successfulness in propagation. Semi hardwood and hardwood cuttings of F. leucopyrus were well established within a period of one week in sand potting medium while softwood cutting has not responded in any of the media during the period. Medium with only coir dust was also not successful in establishing the cuttings.