ICAPS-2021

Permanent URI for this collectionhttp://repository.kln.ac.lk/handle/123456789/23880

Browse

Now showing 1 - 2 of 2

Antimicrobial activity of selected plant extracts against Salmonella typhi and Escherichia coli and their phytochemical screening
(Faculty of Science, University of Kelaniya, Sri Lanka., 2021) Wijekoon, W. M. A. N.; Gayathri, S. M. D. S.; Dias, P. G. I.; Anuradha, N. G. D.; Kananke, T. C.; Athnayaka, R. M. K. T.; Perera, M. G. A. N.; Sabaragamuwa, R. S.; Wickramaratne, M. N.; Rathnayaka, R. M. U. S. K.
Salmonella typhi and Escherichia coli are foodborne pathogens that cause food poisoning, various infections, and sepsis. Emergence of microbial resistance to currently available antibiotics creates a notable curiosity to find novel antibacterial drugs or complementary medicines. Olax zeylanica L. (Mella), Phyllanthus debilis (Ela pitawakka), Osbeckia octandra (Heen bowitiya) and Artocarpus heterophyllus (Kos) leaves are edible remedies used by local villagers. All four plants are abundantly utilized in ayurvedic medicine against different ailments. This study aims to evaluate the antimicrobial activity and screen the phytochemicals in these plant leaves. Initially, methanol and ethanol leaf extracts were prepared and then they were concentrated using the rotary evaporator. Antibacterial activity of the plant extracts (10 mg/ml) against S. typhi and E. coli was evaluated using the well diffusion method (40 µl/well) in Mueller Hinton agar and replicated thrice. Positive (Ciprofloxacin) and negative controls (ethanol and methanol) were also tested. The size of the microbial inoculum was adjusted to the cell count of 6.0 ×108 CFU/ml, approximately using the McFarland standard. Preliminary phytochemical screening was conducted for flavonoids, saponins, steroids, tannins, and terpenoids using standard techniques. Statistical analysis was conducted using two-way ANOVA and Fisher LSD method. The highest average diameter of the inhibitory zone against E. coli was recorded for the methanol extract of O. zeylanica (0.94 ± 0.02 mm) while the lowest was for A. heterophyllus ethanol extract (0.68 ± 0.02 mm). The methanol extract of P. debilis showed the highest average diameter of the inhibitory zone (1.79 ± 0.08 mm) against S. typhi while O. zeylanica ethanol extract showed the lowest diameter (0.59 ± 0.03 mm). The diameters were significantly different (P<0.05) among the four plants and different extract types of the same plant. Tannin was detected in O. octandra, A. heterophyllus and P. debilis. Saponin was detected only in O. zeylanica. Terpenoids were detected in all other plants except in P. debilis. Further analysis with more advance tests is needed to confirm the presence and quantity of phytochemicals with higher accuracy. Since all plants exhibited antibacterial activities, identification of the specific chemical compounds responsible is important for synthesizing plant-based antimicrobials.
Physicochemical, techno-functional and hypoglycemic properties of selected wheat flour substitutes
(Faculty of Science, University of Kelaniya, Sri Lanka, 2021) Ranasinghe, M. M. K. D.; Gayathri, S. M. D. S.; Dias, P. G. I.; Anuradha, N. G. D.; Kananke, T. C.; Perera, M. G. A. N.; Rathnayaka, R. M. K. T.; Sabaragamuwa, R. S.; Wickramarathne, M. N.; Rathnayaka, R. M. U. S. K.
Wheat flour is one of the major refined carbohydrate sources consumed by Sri Lankans. However, long term consumption of such refined starch sources is linked with a high prevalence of various non-communicable diseases, such as obesity, heart disease, and type 2 diabetes. Therefore, the consumers are now concerned about the potential of using wheat flour substitutes for healthy food recipes. The present study was conducted to find the possibility of utilizing Musa paradisiaca (Ash plantain), Cycas cricinalis (Cycas) and Caryota urens (Kithul) flour types as wheat flour alternatives. The Cycas flour was prepared from the fruits of C. cricinalis and the fruits were sun dried for 3-4 days to remove toxicity before milling. The Kithul flour was extracted from the stem of C. urens. The Ash plantain flour was prepared using well ripened fingers of M. paradisiaca, followed by blanching, drying and milling. The proximate composition, techno-functional and hypoglycemic properties of prepared flour substitutes were determined and compared with wheat flour. The proximate compositions were determined using AOAC approved protocols on a dry matter basis. The pH, water activity, Water Absorption Capacity (WAC), Oil Absorption Capacity (OAC), and swelling properties were determined using appropriate physical and chemical methods. In vitro starch-hydrolyzation method was used to evaluate the Hydrolysis Index (HI), predicted Glycemic Index (GI), and Glycemic Load (GL). Among the flour types considered, the lowest crude fat content was observed in C. urens (1.96 ± 0.23%) while the lowest total carbohydrate content was observed in C. cricinalis (33.15±0.75%). All flour substitutes showed significantly (at P<0.05) less carbohydrate contents compared with that of wheat flour. Further, M. paradisiaca, C. cricinalis, C. urens showed significantly (p<0.05) high fiber contents compared to wheat flour. The WAC and OAC of the three flour substitutes were significantly higher than the wheat flour (at p<0.05). The highest WAC and OAC were observed in C. urens and C. cricinalis, respectively. Although wheat flour reported the highest swelling capacity, it was not significant (at p<0.05) compared to that of other flour types. The lowest predicted HI and GI was observed in M. paradisiaca. However, the HI and GI values (143.93±0.88, 118.73±0.48) of C. urens were higher than that of wheat flour (100±4.55, 94.61±2.5), respectively. The GL values of alternative flour types were significantly (at p<0.05) lower than wheat flour (63.48±1.68) and the value of C. cricinalis (26.68±0.63) obtained the least. Accordingly, C. cricinalis and M. paradisiaca have great potential to be used as a substitute for wheat flour. However, further investigations are to be done to find the best proportions of flour composites to formulate different food items.