International conference on Frontiers in Chemical Technology 2020 (FCT 2020)

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Antioxidant, anti-inflammatory and antibacterial potentials of aqueous leaf extract of Pouteria campechiana (Kunth) Baehni (Ratalawulu)
(Institute of Chemistry Ceylon Adamantane House, Rajagiriya, Sri Lanka., 2020) Hettiarachchi, O. I.; Deraniyagala, S. A.; Hettiarachchi, C. M.
Antioxidants are of dietary importance in preventing oxidative stress due to the production of excess free radicals in our body leading to many diseases including cancer, atherosclerosis and diabetes as well in aging. Despite the advances in modern medicine, plant -based remedies play an important role in healthcare due to less side effects. Due to the side effects of NSAIDS and antibiotics, plant -based remedies would be beneficial. This study was conducted to assess the potential use of P. campechiana (Ratalawulu) as an antioxidant, anti-inflammatory and antibacterial agent. The aqueous leaf extract of Ratalawulu was prepared according to the method of decoction preparation in ayurvedic medicine. All assays were carried out according to previously published procedures. Aqueous leaf extract of P. Baehni showed a Total Phenolic Content of 230.71±7.62 mg (PGE)/g in the Folin-Ciocalteu method. Total flavonoid content was 140.98 ± 98.46 mg (QE)/g according to AlCl3 colorimetric assay [PGE- Pyrogallol equivalence, QE- Quercetin equivalence]. 2,2-Diphenyl-1-picrylhydrazyl radical scavenging activity of aqueous leaf extract of P. Baehni ranged between 1.4%-17.8% for concentration between 0.01-0.1 mg/ml whereas ascorbic acid showed 2.46%-43.93% for the same concentration range. Ferric ion reducing assay for aqueous leaf extract of P. Baehni showed gradual increase in reducing property relating to antioxidant action by donating a hydrogen atom to break free radical chains. Hydroxyl scavenging activity showed 5.73%-44.03% for concentration series of 20-100 mg/ ml whereas, Ascorbic acid ranged from 9.40%-47.06%. Hydroxyl radical is one of the potent reactive oxygen species in the biological system. Nitric oxide scavenging anti-inflammatory assay showed 58.06%-75.36% whereas ascorbic acid showed 80.73%-91.76% for concentration series of 0.4-6.4 mg/ ml. In the human red blood cell assay, percentage protection by aqueous leaf extract of P. Baehni was 15.0%-62.4% and that of ascorbic acid was 12.1%-72.8% for 6.0-300 μg/ml. Aqueous leaf extract of P. Baehni also showed antibacterial activity against Staphylococcus aureus. Taken together, these results suggest the potential use of aqueous leaf extract of P. Baehni as an antioxidant, anti-inflammatory and antibacterial agent, and merit further related investigation.
In vitro antiglycation and antioxidant activity of the aqueous extract of leaves of Solanum torvum
(Institute of Chemistry Ceylon Adamantane House, Rajagiriya, Sri Lanka., 2020) Irfan, F. A.; Deraniyagala, S. A.
Protein Glycation, is a non-enzymatic process which forms Advanced Glycation End Products (AGEs). Accumulation of AGEs in the body causes health complications, especially those related to diabetes. Oxidative stress is also considered to be a contributing factor for such health complications. The present study was undertaken to look into the inhibitory potential on the formation of AGEs as well as the anti-oxidant potential of the aqueous extract of the leaves of the Sri Lankan variety of Solanum torvum (thibbatu) [AELST].The extract was prepared according to the method of Kasaya preparation in Ayurveda medicine. Fructose mediated Protein Glycation was induced by using Bovine Serum Albumin and fructose in buffer of pH 7.4 to mimic AGE formation. The assay showed that AELST has a 7.7% - 85.1% inhibitory effect on AGE formation at a concentration range of 0.25 - 2.0 mg/mL. Amino guanidine which was the positive control, known to have much side effects showed AGE inhibition from 0%- 87.5% for the same concentration range. The plant extract showed the presence of phenols and flavonoids. AELST also showed a radical scavenging activity between 19.2% - 76.7% in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay while the standard BHT showed a percentage inhibition of 13.2% - 83.5% for the concentration range of 25-200 μg/mL. In the hydroxyl radical scavenging assay AELST showed a percentage radical scavenging activity of 29.3% - 42.7% while the positive control ascorbic acid showed a percentage inhibition of 24.1% - 36.9% in a concentration range of 0.5 - 2.0 mg/mL. Hydroxyl radical is one of the potent reactive oxygen species in the biological system. The results show that AELST has the potential to be developed to suppress complications arising due to AGE formation in patients with chronic hyperglycemia and also as a remedy to prevent diseases in which radicals are implicated.