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Item Potential antibacterial activity of selected marine algae against foodborne bacteria(4th International Research Symposium on Pure and Applied Sciences, Faculty of Science, University of Kelaniya, Sri Lanka, 2019) Herath, H. M. B. M. M. D.; Yapa, P. N.; Sandaruwan, M. K. K. D.Bacterial foodborne diseases are becoming a growing public health concern worldwide, increasing the demand of antibiotics. Considering the safety of using natural antimicrobials, the objective of the present study was to evaluate the antibacterial activity of selected marine algae extracts against foodborne bacteria. Whole marine algae samples of Chnoospora minima, Gracilaria foliifera, Gracilaria hikkaduwensis, Ulva prolifera, Sargassum polycystum, and Ulva lactuca were collected from west sea coast of Sri Lanka. Crude algal extracts of distilled water, methanol, acetone and diethyl ether were separately tested for antibacterial activity against Staphylococcus aureus NCTC 6571, Staphylococcus aureus ATCC 25923, Escherichia coli NCTC 10418, E. coli ATCC 25922 and Enterococcus faecalis. Antibacterial activity was evaluated by using the standard well-diffusion method. Each tested strain was suspended in 3 ml of sterile distilled water with a turbidity optically comparable to that of the 0.5 McFarland standard (1.5 × 108 CFU/ ml) and 100 μl aliquots of each suspension were inoculated and uniformly spread on the surface of Muller Hinton agar plates in triplicates separately. After placing 50 μl of extracts into each well, plates were incubated at 37 °C for 24 - 48 hours and the diameter of the growth inhibition zone around the wells were measured. Further, minimum inhibition concentrations of each extract were also evaluated. Comparisons were performed using one-way ANOVA followed by Tukey’s Pairwise Comparisons. It was revealed that the 0.75 g/ml of G. foliifera in distilled water and 0.05 g/ml methanol, acetone, diethyl ether extracts of G. foliifera exhibited antibacterial activity against Enterococcus faecalis. Further, 0.05 g/ml of acetone extracts of U. proliifera, G. hikkaduwensis, C. minima and U. lactuca showed inhibitory effect against Enterococcus faecalis. Acetone (0.05 g/ml), methanol (0.05 g/ml) and diethyl ether extracts (0.5 g/ml) of G. folifera, S. polycystum inhibited the growth of E. coli NCTC 10418 and E. coli ATCC 25922. Distilled water (0.05 g/ml) and methanol extracts (0.1 g/ml) of G. foliifera, S. polycystum showed inhibitory effect against S. aureus NCTC 6571 and S. aureus ATCC 25923. Further, all tested extracts of C. minima were shown antibacterial activity against S. aureus. Methanolic extracts (0.1 g/ml) of G. hikkaduwensis, U. prolifera and U. lactuca inhibited the growth of S. aureus. Results indicated that the potential of these marine algae to be used in isolation of bioactive compounds responsible for antibacterial activityItem Evaluation of antibacterial potential of tea and different herbal plant extracts against Escherichia coli and Staphylococcus aureus(Research Symposium on Pure and Applied Sciences, 2018 Faculty of Science, University of Kelaniya, Sri Lanka, 2018) Hansanee, K. P. N.; Yapa, P. N.Escherichia coli is the most commonly found bacteria in the human intestinal tract. E. coli is non-pathogenic in normal conditions, but virulent strains such as Escherichia coli O157:H7 can cause gastroenteritis, urinary tract infections, neonatal meningitis etc. Staphylococcus aureus colonizes the human skin as a part of the normal flora and the most common pathogen causing bacterial infections in hospitalized patients. Further, S. aureus causes food borne infections in humans as well as pneumonia, bacteraemia, osteomyelitis, endocarditis, sepsis and toxic shock syndrome. The prevalence of methicillin resistant S. aureus (MRSA) and its ability to resist multiple drugs has posed a serious challenge for disease control. Tea is mainly an infusion of leaves of the plant Camellia sinensis consumed for centuries as a beverage and is valued for its medicinal properties. It was hypothesized that some herbal teas could also be useful in fighting against some bacteria especially enteropathogens. In the present study, C. sinensis tea leaves and plant parts frequently used in herbal tea production, were selected and their aqueous extracts were tested for antimicrobial activity against E. coli and S. aureus. C. sinensis black tea and green tea leaves, flower buds of Syzygium aromaticum, stems of Hemidesmus indicus, flowers of Senna auriculata, Aegle marmelos, plant of Aerva lanata, inner bark of Cinnamomum zeylanicum, and rhizome of Zingiber officinale were purchased from domestic markets and herbalists from Mihinthale, Kandy and Kalutara areas. They were air-dried, ground into powder and extracts were made using boiled distilled water, allow to cool at 370C and tested for its antimicrobial effect against E. coli and S. aureus, using standard agar-gel diffusion inhibition test. The test bacteria were introduced and uniformly spread on the surface of Mueller Hinton agar plates. After placing 20 μl of extracts into each well, plates were incubated at 370C for 24 hr and the diameter of the growth inhibition zone around the wells were measured. Comparisons were performed using one-way ANOVA followed by Duncan multiple-range test. Green tea was found to be most effective, followed by black tea, H. indicus and C. zeylanicum extracts, against S. aureus. The tested aqueous extracts did not show antibacterial activity against E. coli. It can be concluded that green and black teas as well as tea made by infusing specific parts of H. indicus and C. zeylanicum can be used to prevent and control diseases caused by S. aureus. Further, E. coli present in human gastrointestinal tract will not be affected by drinking the tested extracts.