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Item In vitro anti-inflammatory and antioxidant properties of peel extract of selected fruits of the citrus family(4th International Research Symposium on Pure and Applied Sciences, Faculty of Science, University of Kelaniya, Sri Lanka, 2019) Hettiarachchi, H. A. C. O.; Gunathilake, K. D. P. P.; Jayatilake, S.The potential of citrus peel extract in delivering anti-inflammatory and antioxidant properties were evaluated using in-vitro assays. Methanolic extracts of freeze dried peels of Ambul Dodam – Citrus aurantium, Lime – Citrus aurantifolia, Jama naaran – Citrus reticulata, Nas naran – Citrus madurensis, Heen naran - Citrus reticulata were assessed using Lipoxygenase inhibitory assay, Folin-Ciocalteau assay and DPPH radical scavenging assay. The lipoxygenase enzyme inhibition of all citrus varieties were analyzed using two different concentrations 50 μg /mL and 75 μg /mL of peel extracts. The lipoxygenase inhibition levels were within the range of 7.97 % to 23.64 % for 50 μg /mL concentration of peel extracts. The lipoxygenase inhibition levels for 75 μg /mL concentration of peel extract were 6.98 % to 17.99 %. For both concentrations, the highest inhibition (23.64 ± 1.96 % for 50 μg /mL and 17.99 ± 2.48 % for 75 μg /mL) was recorded for Heen Naran. Accordingly, the ascending pattern of percentage lipoxygenase enzyme inhibition for all citrus varieties was: Lime < Jama Naran < Nas Naran < Ambul Dodam < Heen Naran. According to the Folin-Ciocalteau assay the total phenolic contents of Nas Naran and Jama Naran were 471.00 and 1394.00 μg gallic acid equivalent per g dry weight of peel, respectively. DPPH radical scavenging activity of peel extracts of selected citrus fruit varieties ranged from 33.96 to 91.44 %. The percentage inhibition of DPPH radical for citrus peel extracts varied according to the order: Lime < Nas Naran < Heen Naran < Jama Naran < Ambul dodam. High percentage of DPPH radical inhibition may be due to the presence of higher phenolic content. Therefore, it can be concluded that the peels of the evaluated citrus species are having significant antioxidant and anti-inflammatory properties. The discovered potentials can be further studied for effective utilization of peel of citrus fruits in the food industryItem Effect of drying methods on the total flavonoid content, total phenolic content and total antioxidant capacity of five plant leaves with potential use in cosmetics(4th International Research Symposium on Pure and Applied Sciences, Faculty of Science, University of Kelaniya, Sri Lanka, 2019) Gamage, D. G. N. D.; Abeysinghe, D. C.; Wijesekara, R. G. S.; Prathapasinghe, G. A.; Dharmadasa, R. M.; Someya, T.Drying is the most common and fundamental method for post-harvest preservation of medicinal plant materials. It allows the quick conservation of medicinal qualities of plant materials in an uncomplicated manner. However, the instability of some flavonoids and phenolic compounds in medicinal plants which exhibit potent antioxidant activity and wide range of pharmacological properties may indicate a sensitivity to different drying treatments. In this sense, it is important to determine the factors that can preserve these crucial bioactive compounds of plant materials during the drying process. Therefore, the objective of the present study was to determine the effect of hot air oven drying, solar drying and shade drying on bioactive ingredients of five cosmetic potential plant leaves. Leaves of Centella Asiatica (L.) Urb., Senna alata (L.) Roxb., Justicia adhatoda L., Ocimum tenuiflorum L., Hibiscus rosa-sinensis L. were dried to a constant weight using shade drier at 30-35 °C, solar drier at 30-40 °C and hot air oven at 40 °C. Folin- Ciocalteau method, aluminum chloride colorimetric assay and phosphomolybdate assay were employed to analyse the total phenolic content, total flavonoid content and total antioxidant capacity of ethanolic extracts of leaves respectively. All assays were performed in triplicate. Results showed that significantly higher flavonoid content, phenolic content and antioxidant capacity of solar dried leaves of O. tenuiflorum and H. rosa-sinensis in comparison with hot air oven drying and shade dried samples. Solar dried C. asiatica and S. alata leaves showed high flavonoid content and antioxidant capacity while the phenolic content was high in shade dried leaves of S. alata and oven dried leaves of C. asiatica respectively. In contrast, J. adhatoda showed the maximum flavonoid content in shade drying samples, the highest phenolic content in solar dried leaves and the maximum antioxidant capacity in oven dried leaves. Moreover, there were no significant differences (p > 0.05) among drying methods in terms of antioxidant capacity and phenolic content of J. adhatoda. and antioxidant capacity of C. asiatica. Thus, it can be concluded that, not only drying temperature and duration but also the plant species and types of metabolites present in plant materials determine the effect of drying method on bioactive compounds. Finally, solar drying of medicinal plant materials using solar drier would be an economical, efficient and effective drying method for preserving bioactive compounds present in leaves of above-mentioned plant materials