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    Effect of Croton aromaticus leaf extracts in controlling Crown Rot disease of Embul banana.
    (International Research Symposium on Pure and Applied Sciences, 2017 Faculty of Science, University of Kelaniya, Sri Lanka., 2017) Dilhani, S.; Wimalasiri, S.; Abeywickrama, K.; Kannangara, S.
    Embul banana (Musa acuminata-AAB) is one of the major dessert fruits in Sri Lanka, widely consumed by all economic groups due to its small size and characteristic flavour. Although Embul banana has high potential for export market storage and export of this commodity over long distances is difficult in consequence of postharvest fungal diseases. The most common and serious postharvest disease that affect Embul banana is Crown Rot (CR). Use of synthetic fungicides is the widely used method in controlling postharvest diseases of fruits worldwide. The interest of finding natural bioactive components has increased due to the harmful effects of synthetic fungicides on environment and health. In the present study, antifungal activity of aqueous, hot water and ethanolic leaf extracts of Croton aromaticus in controlling CR disease of Embul banana was investigated in vivo. Embul banana hands (85-days mature) were treated with C. aromaticus aqueous, hot water 100% (v/v) and ethanolic leaf extracts (400 mg/ml) alone or in combination with alum (1%) or distilled water (control) were stored in modified atmosphere packaging at 12-14 0C for 14 days. Each treatment comprised of 4 replicates. In-package gases were analysed on initial day and thereafter up to 14 days. Physicochemical properties (pH, firmness, TSS, TA), sensory properties (peel colour, flesh colour, aroma, flavour, taste, overall acceptability), and Crown Rot disease severity were determined in ripening induced fruits after 7 and 14 days of storage period. Statistical analysis was done using the MINITAB 16 statistical package. Oxygen levels measured were observed to be amaintained at 2.2-4.4% while CO2 levels were maintained at 5.5-8.4% in all packages at the end of 14 day storage period. C. aromaticus ethanolic leaf extract (400 mg/mL) was the most effective extract in controlling crown rot disease of Embul banana compared to aqueous and hot water leaf extracts. Physicochemical properties of Embul banana treated with C. aromaticus leaf extract alone and in combination with alum were not significantly different compared to control except for TSS and TA. Most of the sensory properties were preferred by sensory panelists with score values of above 6 indicating the good quality of samples. C. aromaticus ethanolic leaf extract + alum in combination with modified atmosphere packaging and cold storage could be used as a potential safe way of controlling Crown Rot disease of Embul banana.
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    Basil oil plus aluminium sulfate and modified atmosphere packagingcontrols Crown rot disease in Embul banana (Musa acuminata, AAB) during cold storage
    (Elsevier, 2017) Siriwardanaa, H.; Abeywickrama, K.; Kannangaraa, S.; Jayawardena, B.; Attanayake, S.
    tEffect of basil oil spray treatment in combination with modified atmosphere packaging was investigatedin controlling crown rot and extending the shelf life of Embul banana (Musa acuminata, AAB) at 12–14◦C.Embul banana fruits were treated with 1% aluminium sulfate, 1% aluminium sulfate + 0.4% basil oil and dis-tilled water (control). Treated banana samples were packed in Low Density Polyethylene bags and storedat 12–14◦C. In-package gases were analysed after 14 days of cold storage. Physicochemical properties,nutritional properties, sensory properties and crown rot disease severity as well as residues in treatedbanana peel were determined in ripening induced fruits. Test marketing trials were conducted in fruit out-lets in Dambulla and Kiribathgoda, Sri Lanka where treatments were provided to consumers and staff toobtain feedback on the quality of treated banana. At the end of 14 days, O2in packages remained between3.1–3.7% while CO2varied from 4.2 to 4.7%. 1% aluminium sulfate + oil treatment significantly controlledcrown rot disease compared to others. Most of the physicochemical, sensory properties of aluminiumsulfate + basil oil treated banana were not significantly different compared to control whereas nutritionalproperties of treated samples showed no drastic changes compared to control. Treated samples scoredhigher for peel colour and taste over control samples, by staff and consumers of fruit outlets. Consumerspreferred basil oil treated banana over untreated due to their sweet and pleasant taste. Insignificantamount of residues persisted in treated banana. This current safe, eco-friendly treatment strategy couldbe recommended in preparing Embul as well as other banana varieties belonging to Musa acuminata, AABgroup for commercial scale export to various destinations which require a transit time of two weeks.